Protein Info for DZA65_RS04260 in Dickeya dianthicola ME23

Annotation: GDP-mannose pyrophosphatase NudK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 194 PF27456: NudK_N" amino acids 5 to 42 (38 residues), 57.6 bits, see alignment 8.2e-20 TIGR00052: nudix-type nucleoside diphosphatase, YffH/AdpP family" amino acids 6 to 185 (180 residues), 201 bits, see alignment E=6.7e-64 PF00293: NUDIX" amino acids 45 to 178 (134 residues), 39.1 bits, see alignment E=7.2e-14

Best Hits

Swiss-Prot: 81% identical to NUDK_PECAS: GDP-mannose pyrophosphatase NudK (nudK) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: None (inferred from 98% identity to ddd:Dda3937_01397)

MetaCyc: 66% identical to GDP-mannose hydrolase (Escherichia coli K-12 substr. MG1655)
3.6.1.-

Predicted SEED Role

"GDP-mannose pyrophosphatase YffH" in subsystem Nudix proteins (nucleoside triphosphate hydrolases)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XUE8 at UniProt or InterPro

Protein Sequence (194 amino acids)

>DZA65_RS04260 GDP-mannose pyrophosphatase NudK (Dickeya dianthicola ME23)
MSSPITILDKQLLSDHWFVLHKYVFDLKRKNGGIVRQMREVYDRGNGAAILLYNRVRGTV
VLTRQFRMPTYLNGNESGMLLEACAGLLDDQSPEQCIRNEAIEETGYQIGEVEKLFEAYM
SPGGVTELVHFFAAEYDDSQRKTNGGGVDDEDIEVLELPFNDALAMIKDGRIRDGKTIML
LQHAQINGWFANMR