Protein Info for DZA65_RS04240 in Dickeya dianthicola ME23
Annotation: NAD(+) kinase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 87% identical to NADK_PECAS: NAD kinase (nadK) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)
KEGG orthology group: K00858, NAD+ kinase [EC: 2.7.1.23] (inferred from 99% identity to ddd:Dda3937_02546)MetaCyc: 80% identical to NAD kinase (Escherichia coli K-12 substr. MG1655)
2.7.1.M29,2.7.1.23 [EC: 2.7.1.23, 2.7.1.M29]
Predicted SEED Role
"NAD kinase (EC 2.7.1.23)" in subsystem NAD and NADP cofactor biosynthesis global (EC 2.7.1.23)
MetaCyc Pathways
- NAD phosphorylation and dephosphorylation (3/3 steps found)
- NAD phosphorylation and transhydrogenation (2/2 steps found)
- cytosolic NADPH production (yeast) (4/5 steps found)
- NADP biosynthesis (1/1 steps found)
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (6/8 steps found)
- NAD(P)/NADPH interconversion (4/6 steps found)
- mitochondrial NADPH production (yeast) (3/5 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.7.1.23 or 2.7.1.M29
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A3A4CQ63 at UniProt or InterPro
Protein Sequence (292 amino acids)
>DZA65_RS04240 NAD(+) kinase (Dickeya dianthicola ME23) MNRSFNCIGIVGHPRHPNALTTHEMLYHWLRAQGYHVVIEQQIARELQLTDAATGSLSEI GQQADLAVVVGGDGNMLGAARVLSRYDINVIGINRGNLGFLTDLDPDHTQQQLSEVLAGR YIREQRFMLEAQVCRANHPNSSSTAINEVVLHPGKVAHMIEFEVYIDDKFAFSQRSDGLI ISTPTGSTAYSLSGGGPILTPSLDAIALVPMFPHTLSARPLVINSSSAIRLKFSHITQDL EISCDSQISLPVQEGEEVLIQRSQYHLNLIHPENYSYFNTLSSKLGWSKKLF