Protein Info for DZA65_RS03910 in Dickeya dianthicola ME23

Annotation: lytic murein transglycosylase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF13406: SLT_2" amino acids 61 to 357 (297 residues), 299.9 bits, see alignment E=1.8e-93 TIGR02282: lytic murein transglycosylase B" amino acids 66 to 360 (295 residues), 381.5 bits, see alignment E=1.1e-118

Best Hits

Swiss-Prot: 69% identical to MLTB_ECOLI: Membrane-bound lytic murein transglycosylase B (mltB) from Escherichia coli (strain K12)

KEGG orthology group: K08305, membrane-bound lytic murein transglycosylase B [EC: 3.2.1.-] (inferred from 97% identity to ddd:Dda3937_01212)

MetaCyc: 69% identical to membrane-bound lytic murein transglycosylase B (Escherichia coli K-12 substr. MG1655)
4.2.2.f [EC: 4.2.2.f]; 4.2.2.f [EC: 4.2.2.f]

Predicted SEED Role

"Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-)" in subsystem Peptidoglycan Biosynthesis (EC 3.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 4.2.2.f

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XTN9 at UniProt or InterPro

Protein Sequence (367 amino acids)

>DZA65_RS03910 lytic murein transglycosylase B (Dickeya dianthicola ME23)
MRRLVAVFPLLLALSACSNKSTEPAGAEIIGTPAANAPSGGFLLSPAHAGGLLTSGDFAN
SPEVDRFVDKMVRQYGFERQQLHDVLAQAQRLDWVIRLMDKQAPSPSTSTPSNIPNGAWL
RYRKQFITPDNVQNGVAFWNQYQDALDRARQVYGVPPEIIVGIIGVETRWGRVMGKTRIL
DALATLAFAYPRRAEYFQSELEYFLLMAREDGFDPLSLRGSFAGAMGYGQFMPSAFKKYA
VDFNGDGIANLWDPVDAIGSVANYFKSNGWQPDGQVAVTASGQTFALETGFKTRYSLSTL
AAAGLRPSAALGGVQEASLLRLDMGSYYQFWYGLPNFYAITRYNHSVHYAMAVWQLGEEV
RKARQGY