Protein Info for DZA65_RS03685 in Dickeya dianthicola ME23

Annotation: cation/acetate symporter ActP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 552 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 34 to 55 (22 residues), see Phobius details amino acids 77 to 99 (23 residues), see Phobius details amino acids 105 to 124 (20 residues), see Phobius details amino acids 150 to 169 (20 residues), see Phobius details amino acids 181 to 202 (22 residues), see Phobius details amino acids 211 to 231 (21 residues), see Phobius details amino acids 264 to 288 (25 residues), see Phobius details amino acids 300 to 325 (26 residues), see Phobius details amino acids 358 to 386 (29 residues), see Phobius details amino acids 407 to 426 (20 residues), see Phobius details amino acids 432 to 455 (24 residues), see Phobius details amino acids 466 to 487 (22 residues), see Phobius details amino acids 501 to 520 (20 residues), see Phobius details TIGR02711: cation/acetate symporter ActP" amino acids 5 to 552 (548 residues), 1113.5 bits, see alignment E=0 PF00474: SSF" amino acids 65 to 471 (407 residues), 450.7 bits, see alignment E=2.3e-139 TIGR00813: transporter, solute:sodium symporter (SSS) family" amino acids 65 to 471 (407 residues), 217.9 bits, see alignment E=2.5e-68

Best Hits

Swiss-Prot: 87% identical to ACTP_PECAS: Cation/acetate symporter ActP (actP) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K14393, cation/acetate symporter (inferred from 99% identity to ddd:Dda3937_02261)

MetaCyc: 83% identical to acetate/glycolate:cation symporter (Escherichia coli K-12 substr. MG1655)
RXN0-1981; RXN0-5111; TRANS-RXN0-576

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CL35 at UniProt or InterPro

Protein Sequence (552 amino acids)

>DZA65_RS03685 cation/acetate symporter ActP (Dickeya dianthicola ME23)
MKSRIFLLTALLGLPVWAQAADAVSGNVQKQPLNIQAIALFVLFVAATLYITYWASKRTR
SRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVYTSGFDGLIYSLGFLVGWPIIL
FLIAERLRNLGRYTFADVASYRLQQKPIRTLSACGSLVVVALYLIAQMVGAGKLIQLLFG
LNYHIAVVLVGILMVMYVLFGGMLATTWVQIIKAVLLLCGATFMAIMVLKTVNFSFDSLF
TEATKVHAKGIAIMKPGGLVSDPISALSLGLALMFGTAGLPHILMRFFTVSNAKEARKSV
FFATGFMGYFYFLTFIIGFGAILLVGANPAFKDAAGALIGGNNMAAIHLANAVGGNFFLG
FISAVAFATILAVVAGLTLAGASAVSHDLYSNVIKKGQATERDELKVSKLTVLALGVIAI
LLGILFENQNIAFMVGLAFSIAASCNFPIILLSMYWSKLTTRGAMIGGWAGLLTAIVLMI
LGPTIWVKILGHASPIYPYEYPALFSMLVAFIGIWFFSITDNSLNAVEERGKFKAQFIRS
QTGLGISKGSSH