Protein Info for DZA65_RS01700 in Dickeya dianthicola ME23

Annotation: glutathionylspermidine synthase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 PF03738: GSP_synth" amino acids 12 to 384 (373 residues), 549 bits, see alignment E=3.2e-169

Best Hits

Swiss-Prot: 73% identical to YGIC_ECOL6: Putative acid--amine ligase YgiC (ygiC) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 96% identity to ddd:Dda3937_00724)

MetaCyc: 73% identical to peptide--spermidine ligase YgiC (Escherichia coli K-12 substr. MG1655)
6.3.1.-

Predicted SEED Role

"Similarity with glutathionylspermidine synthase (EC 6.3.1.8), group 1" in subsystem Glutathionylspermidine and Trypanothione (EC 6.3.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XSJ2 at UniProt or InterPro

Protein Sequence (386 amino acids)

>DZA65_RS01700 glutathionylspermidine synthase family protein (Dickeya dianthicola ME23)
MKRIAINERPNWQEKAAEYGFQFHTMYGEPYWCEDAYYQFTLAQIEELEEATAELNQMCL
QVVDKVVNSEALLSKFRIPRHTWDFVRSSWLSRQPALYARLDLAYDGKSPARLLENNADT
PTSLYESAFFQWIWLEDQIKAGQLPQHADQYNSLQEKLIERFEYLRTHHGFGNMHFTCCR
DTEEDRGTVQYLQDCAQEAGVPSQFLYIDEIGLGDRGQFTDLQDHTIGNLFKLYPWEFML
RETFSTKLADAGVRWLEPSWKSVVSNKALLPMLWQMFPNHPNLLPAYFTEDNPPAMSDYV
VKPLFSREGANIQIYQQGKQIAAVDGPYGEEGSIVQQFHPLPRFNDSYTLIGSWLVGDTP
CGIGLREDRELITQDLSRFYPHIILD