Protein Info for DZA65_RS01215 in Dickeya dianthicola ME23

Annotation: phosphomethylpyrimidine synthase ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 41 to 122 (82 residues), 108.2 bits, E=1.5e-35
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 161 to 613 (453 residues), 739.8 bits, E=4e-227
• PF01964: ThiC_Rad_SAM
  • amino acids 162 to 610 (449 residues), 624.7 bits, E=7.2e-192

Best Hits

Swiss-Prot: 87% identical to THIC_PECAS: Phosphomethylpyrimidine synthase (thiC) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 96% identity to ddd:Dda3937_00235)

MetaCyc: 87% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

See A0A385XS72 at UniProt or InterPro

Protein Sequence (655 amino acids)

>DZA65_RS01215 phosphomethylpyrimidine synthase ThiC (Dickeya dianthicola ME23)
MSTEPKQSTAQNVSGEKTASGRREQRAAAQQFIDTLRGSTFPNSQRIYLTGSQPDIRVPM
REIQLSPTLVGGNRDNPRYEANEAIPVYDTAGPYGDPAVTPDVHRGLSKLRAGWIAERDD
TEALSRVSSSFTQQRLADIGLDHLRFEHLPQPKRAKAGRRVTQLYYARQGIITPEMEFIA
IRENMGRERVRGEVLRQQHPGHGFGALLPDNITPEFVRQEVAAGRAIIPANINHPESEPM
VIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWATRWGADTVMDLSTGRYIHETREWILRN
SPVPIGTVPIYQALEKVNGIAENLTWERFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAK
RLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVALSLGDGLRPGSIQDAND
EAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLT
TDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKNDVKQGLIAYKIAAHAADL
AKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPETARAYHDETLPQESGKVAHFCSMCGP
KFCSMKITQEVRDYAARQEAQAQAADAGMAQMSAEFRARGSELYHTATSLPQEER