Protein Info for DZA65_RS01085 in Dickeya dianthicola ME23

Annotation: UDP-N-acetylmuramate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 67% identical to MURB_SALCH: UDP-N-acetylenolpyruvoylglucosamine reductase (murB) from Salmonella choleraesuis (strain SC-B67)

KEGG orthology group: K00075, UDP-N-acetylmuramate dehydrogenase [EC: 1.1.1.158] (inferred from 91% identity to ddd:Dda3937_04216)

MetaCyc: 65% identical to UDP-N-acetylenolpyruvoylglucosamine reductase (Escherichia coli K-12 substr. MG1655)
UDPNACETYLMURAMATEDEHYDROG-RXN [EC: 1.3.1.98]

Predicted SEED Role

"UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158)" in subsystem Peptidoglycan Biosynthesis or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 1.1.1.158)

MetaCyc Pathways

peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
peptidoglycan recycling I (13/14 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.158 or 1.3.1.98

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CCI4 at UniProt or InterPro

Protein Sequence (345 amino acids)

>DZA65_RS01085 UDP-N-acetylmuramate dehydrogenase (Dickeya dianthicola ME23)
MTNSVTSLKSFNSFSLSVFATEAATVKNSSELLSVWQRAAQGGLPILILGEGSNVLFLDD
FHGIVLVNRLKGIEIKETTSEWMLHVGAGENWHYLVEYTQERQIAGLENLALIPGCVGSA
PIQNIGAYGVELKQVCAYVDMLNLRTGETARLSAEECRFGYRDSVFKHEYQDGFAIIAVG
LRLSKEWKPVLEYGDLTRLDPSAVTAKQVFDAVCQMRRSKLPDPAVMGNAGSFFKNPVIP
AAIAEHILASYPNAPHYPQLNGEVKLAAGWLIDQCGLKGYQIGQAAVHDKQALVLVNKGE
ATSRDLVNLARYVRNRVAEQFEVWLEPEVRFIAAQGEVNALEVLA