Protein Info for DZA65_RS00875 in Dickeya dianthicola ME23

Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 5 to 312 (308 residues), 420.2 bits, E=6.6e-130
  • amino acids 546 to 684 (139 residues), 27.3 bits, E=2.7e-10
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 8 to 752 (745 residues), 1119.3 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 426 to 748 (323 residues), 539.2 bits, E=3.9e-166

Best Hits

Swiss-Prot: 91% identical to METE_PECAS: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 98% identity to ddd:Dda3937_02053)

MetaCyc: 86% identical to cobalamin-independent homocysteine transmethylase (Escherichia coli K-12 substr. MG1655)
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase. [EC: 2.1.1.14]

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis I (5/5 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• S-adenosyl-L-methionine salvage II (2/3 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.14

Use Curated BLAST to search for 2.1.1.14

Sequence Analysis Tools

See A0A3A4CKW0 at UniProt or InterPro

Protein Sequence (756 amino acids)

>DZA65_RS00875 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase (Dickeya dianthicola ME23)
MAILNHTLGFPRVGLRRELKKAQESYWAGNSTQEELLAVGRELRARHWQQQKDAGVEVLP
VGDFAWYDHVLTTSLLLGNVPARHQNADGSVGLDTLFRIGRGRAPTGEPAAAAEMTKWFN
TNYHYMVPEFTKGQQFKLTWTQLLDEVDEALALGHKVKPVLLGPVTYLWLGKVKGEAFNR
LDLLNALLPVYQQVLAELAKRGIEWVQIDEPLLALELDAEWQAAFKPAYDALQGQVKLLL
TTYFDSIGQNLDVIKALPVQGLHIDLVHGKDDAAALNAQLPADWVLSLGVINGRNVWRAD
LASWFERLQPLLGKRTLWLGSSCSLLHSPIDLSVETRLDEEVKSWFAFAIQKCQELALLT
RALNSGNGDELVAYSAPIRARRTSTRVNNPNVAKRLAAITAQDSLRQNPYPVRAQAQRAR
FNLPAWPTTTIGSFPQTTEIRSLRLDFKQGRLDGQNYRIGIAEHIKQAIVEQERLELDVL
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVVIGDISRPEAITVEWAKYA
QSLTDKPVKGMLTGPVTILCWSFPREDVTRETIARQIALALRDEVADLEQAGIGIIQIDE
PALREGLPLHRSDWTAYLEWAVDAFRLNAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD
VITIETSRSDMELLESFEEFEYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQRIPSERL
WVNPDCGLKTRGWPETRQSLANMVQAAKRLRETASK