Protein Info for DZA65_RS00270 in Dickeya dianthicola ME23

Annotation: mannitol-1-phosphate 5-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 82% identical to MTLD_PECCP: Mannitol-1-phosphate 5-dehydrogenase (mtlD) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00009, mannitol-1-phosphate 5-dehydrogenase [EC: 1.1.1.17] (inferred from 98% identity to ddd:Dda3937_00994)

MetaCyc: 78% identical to mannitol-1-phosphate 5-dehydrogenase (Escherichia coli K-12 substr. MG1655)
Mannitol-1-phosphate 5-dehydrogenase. [EC: 1.1.1.17]

Predicted SEED Role

"Mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17)" in subsystem Mannitol Utilization (EC 1.1.1.17)

MetaCyc Pathways

hexitol fermentation to lactate, formate, ethanol and acetate (19/19 steps found)
superpathway of hexitol degradation (bacteria) (15/18 steps found)
mannitol cycle (4/5 steps found)
mannitol degradation I (1/1 steps found)

KEGG Metabolic Maps

Fructose and mannose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XW92 at UniProt or InterPro

Protein Sequence (382 amino acids)

>DZA65_RS00270 mannitol-1-phosphate 5-dehydrogenase (Dickeya dianthicola ME23)
MKALHFGAGNIGRGFIGKLLADAGISLTFADVNQPLLDAINQRGSYPVRIVGAQQQVETV
SQVSAVHSGSPQVVELIAGVDLVTTAVGPQILAKIAGAIAQGLVERHANGNTSPLNIIAC
ENMVRGTSQLKQHVLTQLPEDTQAWVAQHVGFVDSAVDRIVPPAEAGESDPLAVTVETFS
EWIVDKTQFNGEPPAIAGMELTDNLMAFVERKLFTLNTGHAITAYLGQRAGHGTIRDAIL
DPAIRRVVQGAMEESGAVLIRRYGFDADKHQAYIRKILTRFENPWLHDEVERVGRQPLRK
LSAGDRLVKPLLGTLEYGLPHDNLIQGIAAAMHYRSEQDPQAQELAALIASQGPKVALAQ
ISGLDADREVVGKAVSVYNAMQ