Protein Info for DZA65_RS00195 in Dickeya dianthicola ME23

Annotation: glyoxylate/hydroxypyruvate reductase GhrB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 5 to 315 (311 residues), 97.7 bits, E=4.7e-32
• PF02826: 2-Hacid_dh_C
  • amino acids 104 to 283 (180 residues), 206.7 bits, E=1.8e-65

Best Hits

Swiss-Prot: 75% identical to GHRB_PECAS: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00090, gluconate 2-dehydrogenase [EC: 1.1.1.215] (inferred from 92% identity to dze:Dd1591_0038)

MetaCyc: 68% identical to glyoxylate reductase (Escherichia coli K-12 substr. MG1655)
Glyoxylate reductase (NADP(+)). [EC: 1.1.1.79]; Gluconate 2-dehydrogenase. [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]

Predicted SEED Role

"Glyoxylate reductase (EC 1.1.1.79) / Hydroxypyruvate reductase (EC 1.1.1.81) / 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-)" in subsystem D-gluconate and ketogluconates metabolism or Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.1.-, EC 1.1.1.215, EC 1.1.1.79, EC 1.1.1.81)

MetaCyc Pathways

• ketogluconate metabolism (7/8 steps found)
• L-serine biosynthesis II (4/4 steps found)
• photorespiration II (8/10 steps found)
• formaldehyde assimilation I (serine pathway) (10/13 steps found)
• photorespiration I (7/9 steps found)
• photorespiration III (7/9 steps found)
• L-ascorbate degradation IV (1/5 steps found)
• L-arabinose degradation IV (3/8 steps found)
• D-xylose degradation IV (2/7 steps found)
• superpathway of pentose and pentitol degradation (12/42 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Benzoate degradation via CoA ligation
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of type II polyketide products
• Biosynthesis of unsaturated fatty acids
• Bisphenol A degradation
• Butanoate metabolism
• C21-Steroid hormone metabolism
• Fructose and mannose metabolism
• Glycine, serine and threonine metabolism
• Glyoxylate and dicarboxylate metabolism
• Insect hormone biosynthesis
• Linoleic acid metabolism
• Nucleotide sugars metabolism
• Pentose phosphate pathway
• Polyketide sugar unit biosynthesis
• Pyruvate metabolism
• Retinol metabolism
• Tetrachloroethene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.-

Use Curated BLAST to search for 1.1.1.- or 1.1.1.215 or 1.1.1.79 or 1.1.1.81

Sequence Analysis Tools

See A0A385XTI7 at UniProt or InterPro

Protein Sequence (320 amino acids)

>DZA65_RS00195 glyoxylate/hydroxypyruvate reductase GhrB (Dickeya dianthicola ME23)
MKPEVILYKKIPDDLLKKLEQHATVSVFDGLPPADHPILARVEGLIGAGHTVSREYLSHL
PKLRAVSTVSVGYDNIDLAALNEKKALLMHTPTVLTETVADTVLTLMLMTARRGLESAER
VKAGEWTRNIGEDWFGIDVHHKTIGILGMGRIGLAVAQRVHFGFGMPVLYHARRRHQDAE
TRFNAGYRDLDGLLAESDFLCITLPLTPETHHLIGKAQLAKMKSSAILINIGRGPVVDEQ
ALIEALSDGTLYAAGLDVFEKEPLSVDSPLLKLPNVVALPHIGSATHETRYNMAACAVDN
LIAALSGNVKENCVNPDVLK