Protein Info for DZA65_RS00050 in Dickeya dianthicola ME23

Annotation: ribonuclease Z

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 304 PF23023: Anti-Pycsar_Apyc1" amino acids 1 to 97 (97 residues), 35.6 bits, see alignment E=1.3e-12 TIGR02651: ribonuclease Z" amino acids 2 to 303 (302 residues), 334.8 bits, see alignment E=2.1e-104 PF00753: Lactamase_B" amino acids 35 to 145 (111 residues), 32.3 bits, see alignment E=1.4e-11 PF12706: Lactamase_B_2" amino acids 35 to 150 (116 residues), 36.3 bits, see alignment E=6.6e-13

Best Hits

Swiss-Prot: 67% identical to RBN_ERWT9: Ribonuclease BN (rbn) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K00784, ribonuclease Z [EC: 3.1.26.11] (inferred from 96% identity to ddd:Dda3937_01051)

MetaCyc: 61% identical to ribonuclease BN (Escherichia coli K-12 substr. MG1655)
Ribonuclease Z. [EC: 3.1.26.11]; Exoribonuclease II. [EC: 3.1.26.11, 3.1.13.1]; 3.1.13.1 [EC: 3.1.26.11, 3.1.13.1]

Predicted SEED Role

"Ribonuclease Z (EC 3.1.26.11)" in subsystem tRNA processing (EC 3.1.26.11)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1, 3.1.26.11

Use Curated BLAST to search for 3.1.13.1 or 3.1.26.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CZH2 at UniProt or InterPro

Protein Sequence (304 amino acids)

>DZA65_RS00050 ribonuclease Z (Dickeya dianthicola ME23)
MELIFLGTNAGVPTKERNVTSIALDLHGIRPAFWLFDCGEGTQHQLLRTPVKPGKLEKIF
ITHLHGDHLFGLPGLLCSRSMAGIETPLTLYGPAGLKAFVEATLTLSGSWLTYPLEVVEV
VPGTVFEDHQFRVTAHELSHTLYCVGYRIEERPKPGPLDVDKLAAAGVKPGAYFQQLKRG
ETVTLDDGRVLNGWDYVGPGLPGKSLAIFGDTRPTPEALKLAAGVDVMVHEATLEGALAE
RADARGHSTTLQTAATARDAGAKRLIITHFSARYGQEDLERLRQECQTLFPATEVATDLA
TFRV