Protein Info for DDA3937_RS21365 in Dickeya dadantii 3937

Annotation: 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 113 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 38 to 60 (23 residues), see Phobius details amino acids 67 to 87 (21 residues), see Phobius details amino acids 93 to 112 (20 residues), see Phobius details PF00892: EamA" amino acids 10 to 109 (100 residues), 38.7 bits, see alignment E=5.5e-14

Best Hits

Swiss-Prot: 62% identical to ARNE_ERWT9: Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K12962, undecaprenyl phosphate-alpha-L-ara4N flippase subunit ArnE (inferred from 100% identity to ddd:Dda3937_01075)

MetaCyc: 53% identical to undecaprenyl-phosphate-alpha-L-Ara4N flippase - ArnE subunit (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-276

Predicted SEED Role

"Polymyxin resistance protein PmrL, sucrose-6 phosphate hydrolase" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance )

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SD01 at UniProt or InterPro

Protein Sequence (113 amino acids)

>DDA3937_RS21365 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (Dickeya dadantii 3937)
MTNYLLILLVSLLTCAGQLSQKQAASWQSGGRSRRAHIVLWMGISLLLLGLAMVLWLAVL
QRVAVSVAYPMLSLNFILVALAARWLWREAISLRQSVGIVLIVVGVILMGGIA