Protein Info for DDA3937_RS21235 in Dickeya dadantii 3937

Annotation: adenylosuccinate lyase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00206: Lyase_1
  • amino acids 17 to 297 (281 residues), 177.2 bits, E=5.8e-56
• PF10397: ADSL_C
  • amino acids 365 to 442 (78 residues), 69.9 bits, E=1.9e-23

Best Hits

Swiss-Prot: 46% identical to TAD1_USTMD: Trans-aconitate decarboxylase 1 (TAD1) from Ustilago maydis

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 100% identity to ddd:Dda3937_01048)

MetaCyc: 46% identical to trans-aconitate decarboxylase (Ustilago maydis)
RXN-19902 [EC: 4.1.1.113]

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• itaconate biosynthesis II (1/3 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (1/4 steps found)
• aromatic compounds degradation via β-ketoadipate (2/9 steps found)
• toluene degradation III (aerobic) (via p-cresol) (1/11 steps found)
• superpathway of aerobic toluene degradation (3/30 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (6/35 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.113 or 5.5.1.2

Sequence Analysis Tools

See E0SNE4 at UniProt or InterPro

Protein Sequence (450 amino acids)

>DDA3937_RS21235 adenylosuccinate lyase family protein (Dickeya dadantii 3937)
MASNVLDSILFRDSFGTPEMRAVFDDHELIRKYVEVEIALAKAEARCGVIPQQAADEIAK
TCNADTLDFDLLRHETEIVGYPILPLVHQISKQAGASGGYVHWGATTQDIMDTAVVLQIR
DAFDLIEADIRSLRNTLADLARRYRNTPMAGRTHLQQALPITFGYKAAIWLDMFDRHAER
LEQARPRILVGEFAGAAGTLASLGDNGLAVQKAMMEELKLGVPTSTWHVARDGFAEAVNL
LAVITGSLGKIAYDVMLLASNEFGELYEPFVKGRGASSTMPQKRNPISSELMLACAKGVR
QHAGLMLDAMVQDLERATGPWHAEWIAIPESFVLAAGALNQAKFMLSGLIVDEQAMAKNL
DMTKGLIVAEAVMMGLAPYMGRQDAHDVVYDACRIVNTQGGRLADVLNAMPQVSSRLKPE
LIEQLTDPVNYLGIAPEMVDQVLEKSAKRQ