Protein Info for DDA3937_RS21055 in Dickeya dadantii 3937

Annotation: glycine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 689 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 689 (689 residues), 1044.6 bits, see alignment E=0 PF02092: tRNA_synt_2f" amino acids 6 to 551 (546 residues), 707.9 bits, see alignment E=1.1e-216 PF05746: DALR_1" amino acids 581 to 679 (99 residues), 41.4 bits, see alignment E=1.4e-14

Best Hits

Swiss-Prot: 88% identical to SYGB_PECAS: Glycine--tRNA ligase beta subunit (glyS) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 100% identity to ddd:Dda3937_01010)

MetaCyc: 85% identical to glycine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SNA4 at UniProt or InterPro

Protein Sequence (689 amino acids)

>DDA3937_RS21055 glycine--tRNA ligase subunit beta (Dickeya dadantii 3937)
MTDKTFLVEIGTEELPPKALRTLAESFAANFTAELDAAGLGYQSVNWFAAPRRLALKVAG
LSASQPDREVEKRGPAIAQAFDAEGKPTKAAEGWARGCGITVDQAERLTTDKGEWLLFRA
QVKGEAAQALLPGMVSTALAKLPIPKLMHWGDKETQFVRPVHTVTLLLGDELVPGTVLGI
DSARTIRGHRFMGEPEFTIDNADQYPQILLERGKVLADYEARKAKIKADAEDAARNIGGN
ADLSDSLLEEVTSLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDNSGKLMPN
FIFVANIESKDPQQIIAGNEKVVRPRLADAEFFFNTDRKKRLEDHLPRLETVLFQQQLGT
LRDKTDRIAALAGWVAEQIGADVNHAKRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
SEAEDVAVALNEQYQPRFAGDDLPSSPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGALRIIVEKRLPLDLQTLTEEAVRLYGDKLTNVNVVDDVIEFMLGRFRAWYQEEG
HSVDTIQAVLARRPTRPADFDARVKAVSHFRTLEQAEALAAANKRVSNILAKSTETLNDS
VQAALLKENEEIQLATYVTALTSKLAPWFAEGRYQEALGELAQLREPVDNFFDKVMVNAD
DQQVRINRLTLLNELRNLFLKVADISVLQ