Protein Info for DDA3937_RS20280 in Dickeya dadantii 3937

Annotation: NAD(P)H-flavin reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 233 transmembrane" amino acids 109 to 122 (14 residues), see Phobius details PF00175: NAD_binding_1" amino acids 108 to 211 (104 residues), 88.1 bits, see alignment E=3.2e-29

Best Hits

Swiss-Prot: 76% identical to FRE_SALTY: NAD(P)H-flavin reductase (fre) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K05368, aquacobalamin reductase / NAD(P)H-flavin reductase [EC: 1.16.1.3 1.5.1.29] (inferred from 100% identity to ddd:Dda3937_02071)

MetaCyc: 76% identical to FMN reductase [NAD(P)H] (Salmonella enterica enterica serovar Typhimurium)
FMNREDUCT-RXN [EC: 1.5.1.39]

Predicted SEED Role

"NAD(P)H-flavin reductase (EC 1.5.1.29) (EC 1.16.1.3)" in subsystem Ubiquinone Biosynthesis (EC 1.16.1.3, EC 1.5.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.29

Use Curated BLAST to search for 1.16.1.3 or 1.5.1.29 or 1.5.1.39

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SLH3 at UniProt or InterPro

Protein Sequence (233 amino acids)

>DDA3937_RS20280 NAD(P)H-flavin reductase (Dickeya dadantii 3937)
MTTLSCTVTSVEAITDTVYRVRLLPSAPFSFRAGQYLMVVMDERDKRPFSVASTPIEQGS
IELHIGASEMNLYAMAVMDRILKEKSLVVDIPHGEAWLREDSDRPLILIAGGTGFSYVRS
ILLTVLANQPTRQISVYWGGRELRHLYDLGELQSLAQAHPNLNVIPVVEQPDEQWHGRSG
TVLSAVLQDFGSLAQHDIYIAGRFEMAKIARERFCNERGAQLAHMYSDAFSFI