Protein Info for DDA3937_RS20115 in Dickeya dadantii 3937
Annotation: trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 76% identical to PUTA_ECOLI: Bifunctional protein PutA (putA) from Escherichia coli (strain K12)
MetaCyc: 76% identical to fused DNA-binding transcriptional repressor / proline dehydrogenase / 1-pyrroline-5-carboxylate dehydrogenase PutA (Escherichia coli K-12 substr. MG1655)
RXN0-7008 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]
Predicted SEED Role
"Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)
MetaCyc Pathways
- L-proline degradation I (2/3 steps found)
- proline to cytochrome bo oxidase electron transfer (1/2 steps found)
- L-citrulline biosynthesis (5/8 steps found)
- L-arginine degradation I (arginase pathway) (1/3 steps found)
- superpathway of L-citrulline metabolism (7/12 steps found)
- ethene biosynthesis II (microbes) (1/4 steps found)
- L-Nδ-acetylornithine biosynthesis (3/7 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See E0SKR7 at UniProt or InterPro
Protein Sequence (1329 amino acids)
>DDA3937_RS20115 trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase (Dickeya dadantii 3937) MGTTTMGVKLDEATRERLKAAALRIDRTPHWLIKQAIFSYLERLESGADTPEIPHPAGQD PIEAAEIMPQSPQEETHQPFLDFAEQVLPQSVLRSAITAAYRRPETEMVPMLLEQARMPE AMSHAASMLAAGLAEKLRGQKNSGGRAGMVQSLLQEFSLSSQEGVALMCLAEALLRIPDK PTRDALIRDKISTGNWQSHVGRSASLFVNAAAWGLLVTGKLVATHNESHLSGALNRIISK SGEPLVRKGVDMAMRLMGEQFVTGETIAEALANARKLEERGFRYSYDMLGEAALTGDDAE AYLLSYQQAIHAIGKAAGGRGIYEGPGISIKLSALHPRYGRAQYERVMDELYPRLLSLTL LARQYDIGINIDAEEADRLELSLDLLERLCFEPRLAGWNGIGFVIQAYQKRCPLVIDALI DLARRSQRRLMIRLVKGAYWDSEIKRAQMDGLEGYPVYTRKLYTDVSYLACARRLLAAPN LIYPQFATHNAHTLSAIYHLAGNNYYPGQYEFQCLHGMGEPLYEQVVGKVADGKLNRPCR IYAPVGTHETLLAYLVRRLLENGANTSFVNRIADPSVALETLVADPVSETEALAAREGLV GQPHPRIPLPRALYGKTRRNASGLDLSSEHRLASLSSALLTSAARPWQAQPMLAVEEVNE DDTGDDSRPVVNPAEPRDVVGYVREARPQDIALALEQAVSAGDIWFATPATERAAILSRA ADLLENRQQHLLGLLVREAGKTLTNAVAEVREAVDFLRYYAAQVSETFNNHDYRPLGPVV CISPWNFPLAIFTGQIAAALAAGNSVLAKPAEQTPLIAAQAVQILHEAGVPAGALQLLPG AGETVGAALVSDDRVRGVMFTGSTAVAAQLQRTLAGRLDPQGRTTPLIAETGGINAMIVD SSALTEQVVTDVVTSAFDSAGQRCSALRLLYVQDEVAEKTLAMLRGAMAECRMGNPERLS TDIGPLIDAEAKQRIERHIQTLRAKGRPVFQAAWPNSQDEQEWARGHFIAPTLIELESVE ELRQEVFGPVLHVVRYSRSQLDALIGHINAAGYGLTLGLHTRIDETITRVTSQAKVGNLY VNRNMVGAVVGVQPFGGEGLSGTGPKAGGPLYLCRLLSHRPDDATTRALARQDSERPVDV AARQPLLTGLQALESWARQVQRDELVSCCRRYAELTQSGTVRVLPGPTGERDTYTLLPRE QVLCVADNNDDALVQLAAVLAVGSRALWLDTPERQALYRQLPERVQTRVAFCRDGVTDDS RFDAVIFHGDADRLRHLSEQLAQRAGAIVGVQGLSRGETDIVLERLLIERSLSINTAAAG GNASLMTIG