Protein Info for DDA3937_RS20085 in Dickeya dadantii 3937

Annotation: exopolyphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 498 TIGR03706: exopolyphosphatase" amino acids 8 to 300 (293 residues), 311 bits, see alignment E=4.2e-97 PF02541: Ppx-GppA" amino acids 21 to 301 (281 residues), 341.4 bits, see alignment E=4.9e-106 PF21447: Ppx-GppA_III" amino acids 308 to 481 (174 residues), 123.5 bits, see alignment E=8.3e-40

Best Hits

Swiss-Prot: 85% identical to GPPA_PECCP: Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 100% identity to ddd:Dda3937_00264)

MetaCyc: 74% identical to guanosine-5'-triphosphate,3'-diphosphate phosphatase (Escherichia coli K-12 substr. MG1655)
Guanosine-5'-triphosphate,3'-diphosphate diphosphatase. [EC: 3.6.1.40]

Predicted SEED Role

"Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (EC 3.6.1.40)" in subsystem Phosphate metabolism or Stringent Response, (p)ppGpp metabolism (EC 3.6.1.40)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.11, 3.6.1.40

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SKR1 at UniProt or InterPro

Protein Sequence (498 amino acids)

>DDA3937_RS20085 exopolyphosphatase (Dickeya dadantii 3937)
MPSSSSLYAAIDLGSNSFHMLVVREVAGSVQTLARIKRKVRLAAGLDANNRLSPEAMQRG
WQCLALFSERLQDIPPQQVRVVATATLRLATNADEFLERARQILGLPIQVISGEEEARLI
YQGVAHTTGGPEQRLVVDIGGGSTELATGTGAHHTQLFSLPMGCVTWLERYFTDRDLTQA
HFDRAEQAAREMLRPVKDILRQQGWQVCVGASGTVQALQEIMVAQGMDEYITLGKLRQLK
QRAIQCSKLEELEIEGLTLERALVFPSGLSILLAVFQELEIDSMTLAGGALREGLVYGML
HLPIEQDIRYRTLQNLQRRYLLDSEQALRVRLLADNFLQQVARDWQLDGRCRELLGSACL
IHEIGLSVDFRQAPLHAAYLVRNSDLPGFTPAQKKLLATLLQNQSNTVDPIPLTQQNALP
VNLAQRLCRLLRLAIIFASRRRDDTLPAVRLRVEGESLRVILPAGWLDRHPLRTETLSQE
SLWQSYVHWPLIIEEHTV