Protein Info for DDA3937_RS19940 in Dickeya dadantii 3937

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01891: amidohydrolase
  • amino acids 16 to 364 (349 residues), 355.5 bits, E=1.7e-110
• PF01546: Peptidase_M20
  • amino acids 73 to 376 (304 residues), 141.9 bits, E=2.6e-45
• PF07687: M20_dimer
  • amino acids 181 to 276 (96 residues), 37.4 bits, E=2.2e-13

Best Hits

Swiss-Prot: 51% identical to YXEP_BACSU: Uncharacterized hydrolase YxeP (yxeP) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 100% identity to ddd:Dda3937_00289)

MetaCyc: 51% identical to N-acetyl-sulfur-metabolite deacetylase (Bacillus subtilis subtilis 168)
3.5.1.-

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase homolog (EC 3.5.1.18)" (EC 3.5.1.18)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• S-(2-succinyl)-L-cysteine degradation (3/4 steps found)
• S-benzyl-L-cysteine degradation (2/5 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.18

Use Curated BLAST to search for 3.5.1.18

Sequence Analysis Tools

See E0SKN6 at UniProt or InterPro

Protein Sequence (386 amino acids)

>DDA3937_RS19940 amidohydrolase (Dickeya dadantii 3937)
MKEHAQHAPLAQFIQAFRHDLHQHPELSNQEFETTRKIRAVLEKEGIRILDLPLKTGLVA
EVGGLQDGPLVVVRSDIDALPIEEESGVEFSSKNAGVMHACGHDFHSSAALGAAILLKRI
EPELKGTVRILFQAAEETGLGAPEVIAVGALDGATAIFGIHNDPTLPVGVIGGKDGALTA
GVDRFEIKIAAKGCHAAKPHEGNDPIIILGQLISSVQTIISRTVPSDNNAVVSITQVHSG
STWNVIPDTAYVEGTVRTFSQAVRDLIEQRFRQIVAGIASTFGAEIEFIWHAGPPSVVNT
PRWVDFALQVADDEGFEARRVEASPIGEDFAFYQQKLPGTFMMVGSGGPYALHHPKFRVD
DSALFPTAHYLYQLAKQSLEQLPQTA