Protein Info for DDA3937_RS19830 in Dickeya dadantii 3937

Annotation: lysophospholipase L2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 transmembrane" amino acids 158 to 176 (19 residues), see Phobius details PF12146: Hydrolase_4" amino acids 54 to 312 (259 residues), 186.5 bits, see alignment E=7.6e-59 PF00561: Abhydrolase_1" amino acids 56 to 311 (256 residues), 97.2 bits, see alignment E=2e-31 PF12697: Abhydrolase_6" amino acids 57 to 307 (251 residues), 39.6 bits, see alignment E=1.4e-13

Best Hits

Swiss-Prot: 62% identical to PLDB_ECOLI: Lysophospholipase L2 (pldB) from Escherichia coli (strain K12)

KEGG orthology group: K01048, lysophospholipase [EC: 3.1.1.5] (inferred from 100% identity to ddd:Dda3937_00313)

MetaCyc: 62% identical to lysophospholipase L2 (Escherichia coli K-12 substr. MG1655)
Lysophospholipase. [EC: 3.1.1.5]; 3.1.1.5 [EC: 3.1.1.5]; RXN0-7122 [EC: 3.1.1.5]

Predicted SEED Role

"Lysophospholipase L2 (EC 3.1.1.5)" in subsystem Synechocystis experimental or Triacylglycerol metabolism (EC 3.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5

Use Curated BLAST to search for 3.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SKL1 at UniProt or InterPro

Protein Sequence (330 amino acids)

>DDA3937_RS19830 lysophospholipase L2 (Dickeya dadantii 3937)
MNPYPECLLTREWQYAAFVNGSLLDFWRQRQEGMFIGVDDVPIRFVRFTSPLHQHLVVVL
PGRTDSYVKYAEAAYDLFQCGYDVLMMDHRGQGRSGRLLKDRHRGHVKRFSDYVDDVATL
WQQQVAPGRYTKRFALAHSMGGAILAQFLARQPQAFDAVALCAPMCGILLPMPRWLVWRI
LDWAERYPAIRDYYAIGTSPWRPLPFMANVLTHSHARYRRHVRFYADDPDLRIGGPTYHW
VREALQVETQLLQQAAVIITPLLLLQAEEERVVDNRCQDAFCQALAAAGHPCADGRPYVI
RGARHDILSEKDAMRADAFGRILQHFNDYH