Protein Info for DDA3937_RS19745 in Dickeya dadantii 3937

Annotation: 1,4-alpha-glucan branching protein GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 109 to 723 (615 residues), 991.2 bits, see alignment E=1e-302 PF02922: CBM_48" amino acids 125 to 207 (83 residues), 92.5 bits, see alignment E=2.5e-30 PF00128: Alpha-amylase" amino acids 273 to 341 (69 residues), 32.8 bits, see alignment E=8.9e-12 PF02806: Alpha-amylase_C" amino acids 628 to 724 (97 residues), 86.3 bits, see alignment E=2.2e-28

Best Hits

Swiss-Prot: 99% identical to GLGB_DICCH: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Dickeya chrysanthemi

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to ddd:Dda3937_00330)

MetaCyc: 70% identical to 1,4-alpha-glucan branching enzyme (Escherichia coli K-12 substr. MG1655)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SJV8 at UniProt or InterPro

Protein Sequence (727 amino acids)

>DDA3937_RS19745 1,4-alpha-glucan branching protein GlgB (Dickeya dadantii 3937)
MSGFSDQNIINLLFSGHYADPFAVLGMHDTASGLEVRALLPDASDVWVVDAHSGRKVANL
QCRDPRGFFASAIPRRKKPFSYRLAVTWPQDTQVIDDPYRFGTLLQELDIWLLAEGRHLR
PFETLGAHPSTLDGVVGTCFAVWAPNAQRVSVVGDFNFWDGRRHPMRRRRENGVWELFVP
GVGPGQLYKFEIIDCYGNVLVKSDPYAFESQMRPDTASVVSRLPPALPVDEARQHANDLQ
SPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYVKEMGFTHVELMPVHEHPFDGSWGY
QPLGLYAPTRRFGSPDDFRYLVSAFHEAGINVLLDWVSGHFPADSYGLARFDGTALYEYA
DPKEGYHQDWNTLIYNFDRHEVRNYLAGNALYWTERFGVDGLRVDAVASMIYRDYSRRDG
EWVPNYFGGKENLEAIGFLRYTNQMLGQHHAGAVTIAEESTDYAGVTLPPEHGGLGFHYK
WNMGWMHDSLAYMQLDPVHRKYHHDLLTFGMLYAYSENFVLPLSHDEVVHGKRSLLDRMP
GDVWQKFANLRAYYGFMWAYPGKKLLFMGGEFAQGREWNHDTSLDWHLLDEPEGWHAGVQ
QLVRDLNHCYRQHPPLYQCDYLHQGFEWVVVDDRENSVFAFIRRDADGNEMLIISNFTPV
PRDSYRVGINQPGAWREVLNTDSWHYHGGNLGNKGLVYSETVGSHSRPQSLVLTLPPLAT
LYLVKEA