Protein Info for DDA3937_RS19660 in Dickeya dadantii 3937

Annotation: 4-alpha-glucanotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 728 PF21226: MalQ_N" amino acids 50 to 125 (76 residues), 44.9 bits, see alignment E=1.1e-15 TIGR00217: 4-alpha-glucanotransferase" amino acids 97 to 683 (587 residues), 731.4 bits, see alignment E=4.8e-224 PF02446: Glyco_hydro_77" amino acids 145 to 664 (520 residues), 437.8 bits, see alignment E=5.5e-135

Best Hits

Swiss-Prot: 57% identical to MALQ_ECOLI: 4-alpha-glucanotransferase (malQ) from Escherichia coli (strain K12)

KEGG orthology group: K00705, 4-alpha-glucanotransferase [EC: 2.4.1.25] (inferred from 100% identity to ddd:Dda3937_00347)

MetaCyc: 57% identical to 4-alpha-glucanotransferase (Escherichia coli K-12 substr. MG1655)
4-alpha-glucanotransferase. [EC: 2.4.1.25]; 2.4.1.25 [EC: 2.4.1.25]; 2.4.1.25 [EC: 2.4.1.25]; MALTODEG-RXN [EC: 2.4.1.25]; RXN0-7347 [EC: 2.4.1.25]

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SJU1 at UniProt or InterPro

Protein Sequence (728 amino acids)

>DDA3937_RS19660 4-alpha-glucanotransferase (Dickeya dadantii 3937)
MALKAKKSVPQHPGIADTYKDAYGNEQAIDQETREKLLQLLDVAEPTVAPLPSVCVFRQG
QQNRLPLRDEGEYGWTLTYEKGGIIEGRSTGQAELALPDNLPLGYHQLILTQGEQQWSCR
VIVAPARCYEPDPLTQGKRWWGVMVQLYTLRSSDNWGIGDFGDLKTLVEQVARRGGAFVG
LNPLHALYPAEPEAASPYSPSSRNWLNIIYIDVNQVDDFHQSDAAGEWWKQDDVQRRLTA
ARASRWVDYTAVTSLKLTALRLAFNHFNRRNALDPRKTAFQQFLKTHDESLLQQATYDAL
QAWLKQQGEPAADWLQWPREYHDARSDASLRFRQEHADDVQFYCWLQWLAHEQLAACFSH
SKQLGMPIGLYRDLAVGVAQGGVDTWGDQQLHCMSVTLGAPPDPLGPGGQNWNLTPMHPM
LLRQRGYQPFIDLLRSNMAHSGALRIDHVMGLLRLWWILSGNTATRGAYVLYPVDDLLGI
LALESHRHRCLVIGEDLGTVPEEIVNKLRDNSVYSYKVLFFEKDQHDRFRAPDAYPTRSM
ATITTHDLATLRGYWQGVDLTLGKDLGLYPTDALLQQQHDARESAKQGLLDALHEQGLLP
QRVGRNASLTTMSAQLNRGVQRYLADSASALLGLQLEDWLDMATPVNVPGTHQEYPNWRR
KLSRSLDSIFTDRYLERLIRDIDLRRGGPVPSRNRKTAKPAGDETPEETKKEVQKETNKE
AKKDTKKA