Protein Info for DDA3937_RS19100 in Dickeya dadantii 3937

Annotation: shikimate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00507: shikimate dehydrogenase
  • amino acids 6 to 273 (268 residues), 311.3 bits, E=2.4e-97
• PF08501: Shikimate_dh_N
  • amino acids 9 to 91 (83 residues), 91.9 bits, E=3.6e-30
• PF01488: Shikimate_DH
  • amino acids 122 to 193 (72 residues), 42.1 bits, E=1.4e-14
• PF18317: SDH_C
  • amino acids 240 to 266 (27 residues), 36.2 bits, see alignment (E = 5.8e-13)

Best Hits

Swiss-Prot: 66% identical to AROE_PECAS: Shikimate dehydrogenase (NADP(+)) (aroE) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00014, shikimate dehydrogenase [EC: 1.1.1.25] (inferred from 100% identity to ddd:Dda3937_01528)

MetaCyc: 59% identical to shikimate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Shikimate dehydrogenase. [EC: 1.1.1.25]

Predicted SEED Role

"Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 1.1.1.25)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.25

Use Curated BLAST to search for 1.1.1.25

Sequence Analysis Tools

See E0SIT3 at UniProt or InterPro

Protein Sequence (275 amino acids)

>DDA3937_RS19100 shikimate dehydrogenase (Dickeya dadantii 3937)
MSVSQSFAVFGHPIAHSKSPRIHALFAEQMGISLTYERILAPLDGFEDSLKAFFQHGACG
ANVTAPFKERACAAMDELSERARSAGAVNTIQKKVDGSLYGDNTDGIGLLSDLQQHEFIQ
PHDRVLLVGAGGAARGVIQPLLAYGCELVITNRTFARADALAEHFSRYGQVSALPMEKVG
NHSFDLIINATSSGVAGDVPDLPVSLISPDVCCYDMFYQSEPTPFLKWCIRQGAQRYVDG
MGMLVWQAAHAFNLWHGVMPDAVPVIRKMKQELGA