Protein Info for DDA3937_RS18920 in Dickeya dadantii 3937

Annotation: nitrogenase molybdenum-iron protein alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 482 TIGR01282: nitrogenase molybdenum-iron protein alpha chain" amino acids 12 to 478 (467 residues), 850.5 bits, see alignment E=3.2e-260 TIGR01862: nitrogenase component I, alpha chain" amino acids 27 to 472 (446 residues), 623.5 bits, see alignment E=1.8e-191 PF00148: Oxidored_nitro" amino acids 62 to 466 (405 residues), 347.8 bits, see alignment E=3.8e-108

Best Hits

Swiss-Prot: 89% identical to NIFD_KLEPN: Nitrogenase molybdenum-iron protein alpha chain (nifD) from Klebsiella pneumoniae

KEGG orthology group: K02586, nitrogenase molybdenum-iron protein alpha chain [EC: 1.18.6.1] (inferred from 100% identity to ddd:Dda3937_02156)

MetaCyc: 89% identical to [FeMo]-nitrogenase complex dinitrogenase component alpha subunit (Klebsiella pneumoniae)
Nitrogenase (flavodoxin). [EC: 1.19.6.1]

Predicted SEED Role

"Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1 or 1.19.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SI18 at UniProt or InterPro

Protein Sequence (482 amino acids)

>DDA3937_RS18920 nitrogenase molybdenum-iron protein alpha chain (Dickeya dadantii 3937)
MTNATSERNQAIIQEVLEIFPEKARKERKKHMMVTDPAMDGVGKCLVSNRKSQPGVMTVR
GCAYAGSKGVVFGPIKDMAHISHGPIGCGQYSRAGRRNYYTGVSGVNSFSTLNFTSDFQE
KDIVFGGDKKLTKLIEELEQLFPLTKGISIQSECPVGLIGDDIEAVANASRKAIGKPVVP
VRCEGFRGVSQSLGHHIANDVIRDWVLDNREGKPFESTPYDVAIIGDYNIGGDAWASRIL
LEEMGLRVVAQWSGDGTLVEMENTPHVKLNLVHCYRSMNYISRHMEEKHGIPWMEYNFFG
PTKIAESLRKIAAQFDDSIQQNAEAVIARYQAQTEAVIAKYRPRLEGRKVMLYLGGLRPR
HIIGAYEDLGMEIIGTGYEFGHNDDYDRTLPDLKEGTLMFDDASSYELEAFVKALKPDLI
GSGIKEKYIFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMTLNNPSWSELTAPWLK
SA