Protein Info for DDA3937_RS18415 in Dickeya dadantii 3937

Annotation: bifunctional aspartate kinase/homoserine dehydrogenase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 818 PF00696: AA_kinase" amino acids 2 to 284 (283 residues), 166.1 bits, see alignment E=2.8e-52 TIGR00657: aspartate kinase" amino acids 3 to 460 (458 residues), 486.9 bits, see alignment E=3.4e-150 PF13840: ACT_7" amino acids 314 to 376 (63 residues), 34.3 bits, see alignment 4.3e-12 amino acids 394 to 448 (55 residues), 30.5 bits, see alignment 6.7e-11 PF03447: NAD_binding_3" amino acids 472 to 605 (134 residues), 87.7 bits, see alignment E=2.3e-28 PF00742: Homoserine_dh" amino acids 614 to 809 (196 residues), 163.7 bits, see alignment E=9.8e-52

Best Hits

Swiss-Prot: 84% identical to AK1H_SERMA: Bifunctional aspartokinase/homoserine dehydrogenase 1 (thrA) from Serratia marcescens

KEGG orthology group: K12524, bifunctional aspartokinase / homoserine dehydrogenase 1 [EC: 1.1.1.3 2.7.2.4] (inferred from 100% identity to ddd:Dda3937_00394)

MetaCyc: 81% identical to fused aspartate kinase/homoserine dehydrogenase 1 (Escherichia coli K-12 substr. MG1655)
Aspartate kinase. [EC: 2.7.2.4]; Homoserine dehydrogenase. [EC: 2.7.2.4, 1.1.1.3]

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SH30 at UniProt or InterPro

Protein Sequence (818 amino acids)

>DDA3937_RS18415 bifunctional aspartate kinase/homoserine dehydrogenase I (Dickeya dadantii 3937)
MRVLKFGGTSVANAERFARVADIIESNARQGQVATVLSAPAKITNHLVAMIEKTVAKQDI
QPNITEAEVIFADLLQGLAQSQPGFAYDRLKARVDEEFSQLKQVLHGIALLGQCPDSVNA
AIICRGEKLSIAIMEAVFQARGYQVSVIDPVRSLLAQGHYLESTVDIAESTRRIDEQAIP
ADHVILMAGFTAGNDKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV
PDARLLKSMSYQEAMELSYFGAKVLHPRTIAPIAQFQIPCLIKNTENPQAPGTLIGLESN
DNQYPVKGITNLNNMAMINVSGPGMKGMVGMAARVFAAMSRAGISVVLITQSSSEYSISF
CVSQSELPRARKTLEDEFYLELKEGVLEPLDVVQRLAIISVVGDGMRTLRGLSARLFTAL
ASANINIVAIAQGSSERSISVVVNNDVATTGVRVAHQMLFNTNQALDVFVIGVGGVGGAL
LEQIQRQQPWLKQKHIDVRVCGIANSKAMLTNVHGIPMDRWRDELARAREPFSPAALSRL
VKEYHLLNPVIIDCTSSQAVADQYADFLADGFHVVTPNKKANTGTLRYYHQLRQAAAKSR
RKFLYDTNVGAGLPVIENLQNLLNAGDELIRFNGILSGSLSFIFGKLDEGMSLSDATLLA
KEKGFTEPDPRDDLSGMDVARKLLILAREAGYELELDDIQVDSVLPPEYSQGDVASFLAR
LPELDAEFSRRVEAARSEGKVLRYVGKIDDGKCQVTISAVGGNDPLFKVKDGENALAFYS
RYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKVGV