Protein Info for DDA3937_RS18170 in Dickeya dadantii 3937

Annotation: acetolactate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 4 to 118 (115 residues), 138 bits, E=2.1e-44
• TIGR00118: acetolactate synthase, large subunit, biosynthetic type
  • amino acids 4 to 553 (550 residues), 661.7 bits, E=4.2e-203
• PF00205: TPP_enzyme_M
  • amino acids 192 to 326 (135 residues), 160.4 bits, E=3.1e-51
• PF02775: TPP_enzyme_C
  • amino acids 382 to 529 (148 residues), 175.8 bits, E=8e-56

Best Hits

Swiss-Prot: 71% identical to ILVB_ECOLI: Acetolactate synthase isozyme 1 large subunit (ilvB) from Escherichia coli (strain K12)

KEGG orthology group: K01652, acetolactate synthase I/II/III large subunit [EC: 2.2.1.6] (inferred from 100% identity to ddd:Dda3937_01361)

MetaCyc: 71% identical to acetohydroxy acid synthase I subunit IlvB (Escherichia coli K-12 substr. MG1655)
Acetolactate synthase. [EC: 2.2.1.6]; 2.2.1.6 [EC: 2.2.1.6]

Predicted SEED Role

"Acetolactate synthase large subunit (EC 2.2.1.6)" in subsystem Acetoin, butanediol metabolism or Branched-Chain Amino Acid Biosynthesis (EC 2.2.1.6)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• superpathway of (R,R)-butanediol biosynthesis (5/5 steps found)
• L-valine biosynthesis (4/4 steps found)
• superpathway of L-threonine metabolism (14/18 steps found)
• pyruvate fermentation to (R)-acetoin I (3/3 steps found)
• pyruvate fermentation to (S)-acetoin (3/3 steps found)
• pyruvate fermentation to (R)-acetoin II (2/2 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• superpathway of 2,3-butanediol biosynthesis (4/6 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• L-isoleucine biosynthesis II (4/8 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.6

Use Curated BLAST to search for 2.2.1.6

Sequence Analysis Tools

See E0SGA4 at UniProt or InterPro

Protein Sequence (554 amino acids)

>DDA3937_RS18170 acetolactate synthase large subunit (Dickeya dadantii 3937)
MRYTGAELIIRLLEQQGITTVSGIPGGAALPLYDALGKSRTIRHVLARHEQGAGFIAQGM
ARASGKAAVCMASSGPGATNLLTAIADAKLDSIPLVCITGQVPSGMIGTDAFQEVDTYGI
SIPVTKHNYLVRDISELPQVINDAFRLAQSGRPGPVWVDIPKDVQTATIELAELPAPAEL
TPAPVVDQDALRAAAAMINQAKRPILYLGGGIISAGAHQQAIELAERAGLPTTMTLMALG
AMPVDHPLSLGMLGMHAARYTNMILQQSDLLIVLGARFDDRAIGKAEEFCPDARVIHIDI
DSAEFGKIRQPHVALHADVAQALDQLLPLVDRQARADWLAQVRALQHDFPFSMPNADDPL
SHYGLIRAVAEQLDDQAIITTDVGQHQMWVAQAYPLRRSRQWLTSGGFGTMGFGVPAAIG
AALAEPQRTVVCFSGDGSLMMNIQEMATAVEENLNVKIVLMNNQSLGLVHQQQDLFYQQN
FVASDYRYQTNFLAIAAGFGFATCDLNAAENPQAALRDALQQPGPALIHAAIDINEKVFP
MVPPGAANIEMIGD