Protein Info for DDA3937_RS17480 in Dickeya dadantii 3937

Annotation: tRNA dihydrouridine(20/20a) synthase DusA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 TIGR00742: tRNA dihydrouridine synthase A" amino acids 16 to 332 (317 residues), 539.1 bits, see alignment E=1.9e-166 PF01207: Dus" amino acids 20 to 322 (303 residues), 321.5 bits, see alignment E=2.7e-100

Best Hits

Swiss-Prot: 82% identical to DUSA_ECO57: tRNA-dihydrouridine(20/20a) synthase (dusA) from Escherichia coli O157:H7

KEGG orthology group: K05539, tRNA-dihydrouridine synthase A [EC: 1.-.-.-] (inferred from 100% identity to ddd:Dda3937_03441)

MetaCyc: 82% identical to tRNA-dihydrouridine synthase A (Escherichia coli K-12 substr. MG1655)
1.1.1.-

Predicted SEED Role

"tRNA dihydrouridine synthase A (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SEJ3 at UniProt or InterPro

Protein Sequence (334 amino acids)

>DDA3937_RS17480 tRNA dihydrouridine(20/20a) synthase DusA (Dickeya dadantii 3937)
MKEKESTETLRSNWHGRFSVAPMLDWTDRHCRYFHRLLSHNALLYTEMVTTGAILHGKGD
YLAYSDEEHPLALQLGGSDPAALAQCAVLAEQRGYDEINLNVGCPSDRVQNGRFGACLMA
EAQLVADCVTAMRERVDIPVTVKTRIGIDDQDSYAFLCDFIRTVSEQGGCDTFIVHARKA
WLSGLSPKENREIPPLDYPRVYQLKRDFPSLTLAINGGVKTLAEARQHLQHVDGVMVGRE
AYQNPGMLAQVDRELFGADSAAPDQADVVRAMYPYIERELSAGASLGHITRHMLGLFQGL
PGARQWRRYLSENAHKPGADAAVVERALALVKLV