Protein Info for DDA3937_RS17015 in Dickeya dadantii 3937

Annotation: GTP diphosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 745 PF13328: HD_4" amino acids 48 to 191 (144 residues), 149 bits, see alignment E=2.9e-47 TIGR00691: RelA/SpoT family protein" amino acids 63 to 740 (678 residues), 622.7 bits, see alignment E=4.1e-191 PF04607: RelA_SpoT" amino acids 251 to 360 (110 residues), 144.8 bits, see alignment E=3.5e-46 PF02824: TGS" amino acids 405 to 464 (60 residues), 78.6 bits, see alignment 8.4e-26 PF19296: RelA_AH_RIS" amino acids 483 to 646 (164 residues), 46.4 bits, see alignment E=1.5e-15 PF13291: ACT_4" amino acids 665 to 741 (77 residues), 65.3 bits, see alignment E=2e-21 PF01842: ACT" amino acids 669 to 734 (66 residues), 39.2 bits, see alignment E=1.4e-13

Best Hits

Swiss-Prot: 86% identical to RELA_ECOLI: GTP pyrophosphokinase (relA) from Escherichia coli (strain K12)

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to ddd:Dda3937_00195)

MetaCyc: 86% identical to GDP/GTP pyrophosphokinase (Escherichia coli K-12 substr. MG1655)
GTP diphosphokinase. [EC: 2.7.6.5]; 2.7.6.5 [EC: 2.7.6.5]

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SDL7 at UniProt or InterPro

Protein Sequence (745 amino acids)

>DDA3937_RS17015 GTP diphosphokinase (Dickeya dadantii 3937)
MVAVRSAHLNTEGKFVPDAWIASLGIASKTSCERLAETWRYCEEKTEGHPDAMLLLWRGI
EMVEILSTLSMDNDSMRAAMLFPLADANVVDEDTLRETFGKNIVNLVHGVRDMDAIRQLK
ATQHDSMASEQVDNIRRMLLAMVEDFRCVVIKLAERIAHLREVKDAPEEERVLAAKECTN
IYAPLANRLGIGQLKWELEDFCFRYLHPDEYKRIAKLLHERRIDREQYIDDFVKNLRASM
AQEELKAEVYGRPKHIYSIWRKMQKKSLSFDELFDVRAVRIVVERLQDCYAALGIVHTHY
RHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTLEIQIRTRQMHEDAELGVAAHWKYKEG
AATSGRSGYEERIAWLRKLLAWQEEMADSDEMLDEVRSQVFDDRVYVFTPKGDVVDLPTG
STPLDFAYHIHSDIGHRCIGAKVGGRIVPFTYQLQMGDQIEVITQKQPNPSRDWLNPNLG
YITTSRGRSKIHNWFRKQDRDKNILAGKQILDDELAHLGVSLKMAEKLLLPRYNINSMDE
LLAGIGGGDIRLNQLVNFLQSKVNQPSAEEQDREALRQLTQKSQQPAQRASAKDNGRVVV
EGVGNLMHHIARCCQPIPGDDIIGFITRGRGISIHRADCEQLDDLRSHAPERIVEAVWGE
SYSSGYSLVVRVTANDRSGLLRDITTILANEKVNVLGVSSRSDVKQQLATIDMDIEIYNL
QVLGRVLAKLNQLPDVIDARRLQGN