Protein Info for DDA3937_RS16920 in Dickeya dadantii 3937

Annotation: sulfate adenylyltransferase subunit CysN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 26 to 232 (207 residues), 158.3 bits, E=2.5e-50
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 28 to 434 (407 residues), 681.5 bits, E=4.4e-209
• TIGR00231: small GTP-binding protein domain
  • amino acids 34 to 214 (181 residues), 50.7 bits, E=1.8e-17
• PF03144: GTP_EFTU_D2
  • amino acids 265 to 324 (60 residues), 32.1 bits, E=1.9e-11

Best Hits

Swiss-Prot: 84% identical to CYSN_YERE8: Sulfate adenylyltransferase subunit 1 (cysN) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K00956, sulfate adenylyltransferase subunit 1 [EC: 2.7.7.4] (inferred from 100% identity to ddd:Dda3937_03902)

MetaCyc: 80% identical to sulfate adenylyltransferase subunit 1 (Escherichia coli K-12 substr. MG1655)
Sulfate adenylyltransferase. [EC: 2.7.7.4]

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4)" in subsystem Cysteine Biosynthesis (EC 2.7.7.4)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (2/12 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.7.4

Sequence Analysis Tools

See E0SDJ7 at UniProt or InterPro

Protein Sequence (475 amino acids)

>DDA3937_RS16920 sulfate adenylyltransferase subunit CysN (Dickeya dadantii 3937)
MNHSIAKQIADQGGVEAYLHAQQNKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
TLHNDSKRLGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
TRNMATGASTCELAILLIDARKGVLDQTRRHSFIATLLGIRHLVVAVNKMDLVEYQQAVF
ERFKQDYLDFAGQLPTDLDITFVPISALDGDNVATPSTTMSWYHGPTLLDVLETVNVSSR
SLSQPMRFPVQYVNRPNLDFRGYAGTVASGVVRVGQRVKVLPSGVESSVSRIVTFDGDLQ
QAQAGEAVTLVLSSEVDISRGDLLVGSEETLQPVRSAKVDVVWMAEQPLVPGQSYDIKIA
GKKTRARVENIDYQVEINTLTQRVTESLPLNGIGLVELTFDEPLVLDKYQHNAVTGGMIF
IDRLSNVTVGAGLVREPVELEEAEAGNYSAFELELNALIRRHFPHWGARDLLGGK