Protein Info for DDA3937_RS16615 in Dickeya dadantii 3937

Annotation: acireductone synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01691: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
  • amino acids 2 to 222 (221 residues), 279.6 bits, E=1.6e-87
• PF00702: Hydrolase
  • amino acids 2 to 196 (195 residues), 41.2 bits, E=1.2e-14

Best Hits

Swiss-Prot: 79% identical to MTNC_PECCP: Enolase-phosphatase E1 (mtnC) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K09880, enolase-phosphatase E1 [EC: 3.1.3.77] (inferred from 100% identity to ddd:Dda3937_00922)

MetaCyc: 64% identical to acireductone synthase [multifunctional] (Klebsiella oxytoca)
Acireductone synthase. [EC: 3.1.3.77, 5.3.2.5]; R83-RXN [EC: 3.1.3.77, 5.3.2.5, 3.1.3.87]; 3.1.3.77 [EC: 3.1.3.77, 5.3.2.5, 3.1.3.87]

Predicted SEED Role

"2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatase (EC 3.1.3.77)" in subsystem Methionine Salvage (EC 3.1.3.77)

MetaCyc Pathways

• L-methionine salvage cycle I (bacteria and plants) (11/12 steps found)
• L-methionine salvage cycle III (10/11 steps found)
• L-methionine salvage cycle II (plants) (9/11 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation I (6/7 steps found)

KEGG Metabolic Maps

• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.77 or 3.1.3.87 or 5.3.2.5

Sequence Analysis Tools

See E0SCV9 at UniProt or InterPro

Protein Sequence (230 amino acids)

>DDA3937_RS16615 acireductone synthase (Dickeya dadantii 3937)
MINAIVTDIEGTTSDIRFVHNVLFPYARARLADAVAQAERDPEIAAALTLARQELGQPDA
SPAQLLAAFNQFMDEDRKSPALKLLQGIIWRSGYRNGDFRGHVYDDVAPQLRAWRQQGIA
LYVYSSGSVEAQHLLFGHSDAGDLRPLFSDYFDTRVGAKRETASYHNIARAIGLPACELL
FLSDIRQELDAAELAGWHTCQLIRDDADTDSHHRQVNRFDQIDLNAYQAN