Protein Info for DDA3937_RS16595 in Dickeya dadantii 3937

Annotation: acyl-CoA dehydrogenase FadE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 814 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details transmembrane" amino acids 31 to 59 (29 residues), see Phobius details amino acids 279 to 296 (18 residues), see Phobius details amino acids 582 to 601 (20 residues), see Phobius details PF02771: Acyl-CoA_dh_N" amino acids 145 to 233 (89 residues), 63.7 bits, see alignment E=3.1e-21 PF00441: Acyl-CoA_dh_1" amino acids 360 to 507 (148 residues), 56.7 bits, see alignment E=4.8e-19 PF09317: ACDH_C" amino acids 514 to 792 (279 residues), 388.2 bits, see alignment E=4.5e-120

Best Hits

Swiss-Prot: 75% identical to FADE_YERPE: Acyl-coenzyme A dehydrogenase (fadE) from Yersinia pestis

KEGG orthology group: K06445, acyl-CoA dehydrogenase [EC: 1.3.99.-] (inferred from 100% identity to ddd:Dda3937_00926)

MetaCyc: 73% identical to acyl-CoA dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-17775 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-17796 [EC: 1.3.8.8, 1.3.8.7]; 1.3.8.- [EC: 1.3.8.8, 1.3.8.7]

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2

Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.- or 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SCV5 at UniProt or InterPro

Protein Sequence (814 amino acids)

>DDA3937_RS16595 acyl-CoA dehydrogenase FadE (Dickeya dadantii 3937)
MVAVSVLIVLMLIGALFYHRLSLWQSSALLVLWVVAMAALNLWSAWLLIPLGLLLVPLLA
TPLRQRLISAPALTVFRRVMPPMSKTEKEAIDAGTTWWEGELFRGAPDWHTLHAYPRPAL
TPEEQAFLDGPVEEACRLANDFEITHERADLPPVLWELLKQHRFFALIIKKEYGGLEFSA
YAQAMVLQKLAGVSSILAITVGVPNSLGPGELLQHYGTDEQKDHYLPRLARGDEIPCFAL
TSPEAGSDASAIPDLGVVCYGQWQGQQVLGMRLTWNKRYITLAPVATVLGLAFRLYDPDH
LLGDRDDIGITCALIPTRTEGVKIGRRHFPINIPFQNGPTQGENIFVPLDYIIGGPQMAG
QGWRMLMECLSVGRGITLPSNATGGLKSVALATGAYARIRRQFKLPIGKMEGIEEPLARM
AGNAYVMDAAATLITSGIMQGARPSVLSAIVKYHCTHRGQRGIIDAMDITGGKGICLGPS
NFVARHYQGAPIAITVEGANILTRSMIIFGQGAIRCHPYVLNEMGAAQDNDLKAFDRALF
GHLGHIGGNAMRSLWLGLTNGRTSRAPVSDATRRYYQRLNRLSANLALLADVSMGVLGGS
LKRRERLSARLGDVLSQLYLASATLKRYDEEGRQQADLPLVHWGVQDCLHQAEQAMTELL
RNFPNRLVARLLRAVIFPLGHTSPAPADQLDHQLARLLQAPSATRSRLGRGLYLKATAHH
PAAQLEQSLLDILAAEPIHQRLSQSAGYPLPFMQLDKLAEHGLAEGVITPEEAGVLMQAE
ASRLRSINVDDFAPDALSARKAVAEQRLPADTAA