Protein Info for DDA3937_RS15770 in Dickeya dadantii 3937

Annotation: L-aspartate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 533 transmembrane" amino acids 12 to 25 (14 residues), see Phobius details TIGR00551: L-aspartate oxidase" amino acids 8 to 520 (513 residues), 722.8 bits, see alignment E=1e-221 PF01266: DAO" amino acids 10 to 133 (124 residues), 29 bits, see alignment E=1.7e-10 PF00890: FAD_binding_2" amino acids 10 to 392 (383 residues), 356.4 bits, see alignment E=5.1e-110 PF02910: Succ_DH_flav_C" amino acids 441 to 524 (84 residues), 57.4 bits, see alignment E=3e-19

Best Hits

Swiss-Prot: 83% identical to NADB_YERPE: L-aspartate oxidase (nadB) from Yersinia pestis

KEGG orthology group: K00278, L-aspartate oxidase [EC: 1.4.3.16] (inferred from 100% identity to ddd:Dda3937_03843)

MetaCyc: 79% identical to L-aspartate oxidase (Escherichia coli K-12 substr. MG1655)
1.5.98.-; L-aspartate oxidase. [EC: 1.4.3.16]

Predicted SEED Role

"L-aspartate oxidase (EC 1.4.3.16)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 1.4.3.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SBI1 at UniProt or InterPro

Protein Sequence (533 amino acids)

>DDA3937_RS15770 L-aspartate oxidase (Dickeya dadantii 3937)
MSSIKDYHCDVLIIGSGAAGLSLALRLAPHARVMVLSKGPLSESATFYAQGGIAAVFDET
DSVDAHIDDTLIAGDGLCDREAVSFIARNARHCVQWLIDQGVRFDTETAPHGEARYHLTR
EGGHSHRRILHAADATGKAVETTLVSQVRQHPNIVLLERCNAVDLITSSKLSLPGHKRVV
GAYLWNREKEHVESCRAKAVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANMEF
NQFHPTCLYHPQARNFLLTEALRGEGAWLTRPDGSRFMPAFDKRAELAPRDIVARAIDHE
MKRLGADCMYLDISHKPADFITQHFPTIHEKLLSLGLDLTREPIPVVPAAHYTCGGVVVD
QQGRTDLDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAGDILQHLPEKALVESLP
RWDESQVENSDERVVIQHNWHELRLLMWDYVGIVRTTKRLERAQRRISLLQQEISEYYAH
FRLSNNLLELRNLVQVAELIVRCALARKESRGLHYTLDYPQQAEKAHPTILHP