Protein Info for DDA3937_RS15655 in Dickeya dadantii 3937

Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1294 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 3 to 1292 (1290 residues), 2036.9 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 149 (115 residues), 152.9 bits, see alignment E=6.8e-49 PF18072: FGAR-AT_linker" amino acids 170 to 219 (50 residues), 83.2 bits, see alignment (E = 3e-27) PF02769: AIRS_C" amino acids 431 to 586 (156 residues), 117.7 bits, see alignment E=1.1e-37 amino acids 830 to 964 (135 residues), 50.2 bits, see alignment E=6.8e-17 PF13507: GATase_5" amino acids 1038 to 1291 (254 residues), 364.8 bits, see alignment E=4e-113

Best Hits

Swiss-Prot: 82% identical to PUR4_ECO57: Phosphoribosylformylglycinamidine synthase (purL) from Escherichia coli O157:H7

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 72% identity to aap:NT05HA_1480)

MetaCyc: 82% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SB00 at UniProt or InterPro

Protein Sequence (1294 amino acids)

>DDA3937_RS15655 phosphoribosylformylglycinamidine synthase (Dickeya dadantii 3937)
MEILRGSPALSAFRINKLLARCKEYHLPVSDIYAEYVHFADVNAPLNHEEQSRLSRLLKY
GPSLAEHEPTGRLLLVTPRPGTISPWSSKATDIAHNCGLQKIRRLERGLAFYIHAPTLSD
AQWRELAALLHDRMMESVFDDLQQANLLFSQHQPAPLKRVEILLQGRPALEEANLRLGLA
LADDEMDYLLDAFTKLGRNPTDIELYMFAQANSEHCRHKIFNADWVINGETQPKSLFKMI
KNTFEHTPDYVLSAYKDNAAVMEGSAVGRFFADPQGAYGYHQEDAHILMKVETHNHPTAI
SPWPGAATGSGGEIRDEGATGRGAKPKAGLVGFSVSNLRIPGFIQPWEQDFGKPDRIVSA
LDIMTDGPLGGAAFNNEFGRPALTGYFRTYEEVVDSHNGVEVRGYHKPIMLAGGIGNIRA
GHVKKGEISIGAKLIVLGGPAMNIGLGGGAASSMASGQSDADLDFASVQRDNPEMERRCQ
EVIDRCWQLGEQNPILFIHDVGAGGLSNAMPELVSDGGRGGRFELRDILNDEPGMSPLEV
WCNESQERYVLAVAPEQLTQFDEICRRERAPYAVIGEATEEQHLTLNDRHFNNKPIDMPL
DVLLGKTPKMLRDVERKDVEGTPLNREGIYLAEAVERVLHLPAVAEKTFLITIGDRTVTG
MVARDQMVGPWQVPVADCAVTTASLDSYYGEAMSIGERAPVALRNFAASARLAVGEALTN
IAATHIGELKRVKLSANWMAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMK
TRWQENGEDKSVTAPLSLVISAFARVEDVRHTVTPQLRTDKDNVLLMIDLGAGHHALGAT
ALAQVYRQLGRKTADVRNPAQLAGFFNAMQALVANQALLAYHDRSDGGLLVTLAEMAFAG
HCGVKADIGSMGEDALAVLFNEELGAVIQIDASRRAEVEQVLAEHGLADCVHYLGQAEAG
NHFIIHSGDDVVYHESRTTLRNWWAETTWQMQRLRDNPQCADQEHHAKSDDNDPGLNVAL
TFDLREDIAAPFISRQARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDIHMSDLLAGRRN
LQDFQALVACGGFSYGDVLGAGEGWAKSILFNDRVRDEFAAFFLRPQTLALGVCNGCQMM
SNLRELIPGAEHWPRFVRNKSDRFEARFSLVDVTNSPSLFLQDMAGSRMPIAVSHGEGRV
EVRDDSHLAALEQHQLVALRYVNNYGQATEDYPANPNGSPNGITAVTSASGRATVMMPHP
ERVFRTVSNSWHPEAWGEDSPWMRMFRNARRQLG