Protein Info for DDA3937_RS15510 in Dickeya dadantii 3937

Annotation: aminopeptidase PepB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 PF12404: DUF3663" amino acids 6 to 82 (77 residues), 117.1 bits, see alignment E=2.7e-38 PF00883: Peptidase_M17" amino acids 110 to 422 (313 residues), 354.4 bits, see alignment E=5.8e-110

Best Hits

Swiss-Prot: 84% identical to PEPB_PECCP: Peptidase B (pepB) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K07751, PepB aminopeptidase [EC: 3.4.11.23] (inferred from 100% identity to ddd:Dda3937_03696)

MetaCyc: 76% identical to aminopeptidase B (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; PepB aminopeptidase. [EC: 3.4.13.18, 3.4.11.23]

Predicted SEED Role

"Peptidase B (EC 3.4.11.23)" (EC 3.4.11.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.23 or 3.4.13.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SN83 at UniProt or InterPro

Protein Sequence (431 amino acids)

>DDA3937_RS15510 aminopeptidase PepB (Dickeya dadantii 3937)
MTTEAMLITLSHQPADARWGEKASLTANEQGFTIHLSGDKPLTIIQRAARKIDGQGIKQV
KLAGEGWNLESSWAFWQGYRGPKGKRTVEWATLADAQQQELERRLKIVDWVRDTINLPAE
DLGPEQLATRAIDLLCSVAGEAISYRITKGEDLREQNYAGLHTVGRGSERSPVLLALDYN
PTGKADAPVLACLVGKGITFDTGGYSLKGSSFMDSMKSDMGGAATVTGALALAAARGLQQ
RVKLYLCCADNMVSGNAFRLGDIIRYRNGKTVEVMNTDAEGRLVLADGLIDACEQNPQWI
VDCATLTGAAKTALGNDYHALFSFDDAMIDSLMDSARREGEPFWRLPLEEFHRQHLPSNF
AELNNVAGAAHTAGASTAAAFLSHFVKNYQQGWLHIDCSATYRKSAVEQWSAGATGLGVR
TLADFLLNKAG