Protein Info for DDA3937_RS15495 in Dickeya dadantii 3937

Annotation: 3-mercaptopyruvate sulfurtransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 238 to 259 (22 residues), see Phobius details PF00581: Rhodanese" amino acids 11 to 131 (121 residues), 49 bits, see alignment E=3.5e-17 amino acids 161 to 275 (115 residues), 47.4 bits, see alignment E=1.2e-16

Best Hits

Swiss-Prot: 58% identical to THTM_ECOLI: 3-mercaptopyruvate sulfurtransferase (sseA) from Escherichia coli (strain K12)

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 93% identity to dze:Dd1591_1113)

MetaCyc: 58% identical to 3-mercaptopyruvate sulfurtransferase (Escherichia coli K-12 substr. MG1655)
3-mercaptopyruvate sulfurtransferase. [EC: 2.8.1.2]; Thiosulfate sulfurtransferase. [EC: 2.8.1.2, 2.8.1.1]

Predicted SEED Role

"Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)" (EC 2.8.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.1

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (284 amino acids)

>DDA3937_RS15495 3-mercaptopyruvate sulfurtransferase (Dickeya dadantii 3937)
MSTSAAGLFVSASWLNAHRHDADIALIDARMLPPGNDTRDIAAEYRAAHLPGAVFFDIES
LSDHQTPLPHMMPDIDAFADALGKLGLNEQQHLVIYDEGNLFSAPRAWWMLRLTGASRIS
ILSGGLAGWKQQGLPLEQGDVSPTAQVFHAQTPAAGAIRSLDDVLALCRTGDEQIVDARP
APRFLGEVDEPRPGLRRGHIPGSFNVPWSVLVEGGALKPADELAAIFHQAGVDIQRPIVA
SCGSGVTASVVMLALFVLNAPQVSLYDGSWSEWGARDDVPVVTG