Protein Info for DDA3937_RS15335 in Dickeya dadantii 3937

Annotation: bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00097: hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
  • amino acids 6 to 263 (258 residues), 327.9 bits, E=1.9e-102
• PF08543: Phos_pyr_kin
  • amino acids 13 to 261 (249 residues), 304.9 bits, E=2.1e-95

Best Hits

Swiss-Prot: 77% identical to THID_ECOLI: Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (thiD) from Escherichia coli (strain K12)

KEGG orthology group: K00941, hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [EC: 2.7.1.49 2.7.4.7] (inferred from 100% identity to ddd:Dda3937_02807)

MetaCyc: 77% identical to bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (Escherichia coli K-12 substr. MG1655)
Phosphomethylpyrimidine kinase. [EC: 2.7.4.7]; Hydroxymethylpyrimidine kinase. [EC: 2.7.4.7, 2.7.1.49]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7)" (EC 2.7.4.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• thiamine diphosphate salvage II (5/5 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• hydroxymethylpyrimidine salvage (2/2 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (5/8 steps found)
• thiamine diphosphate salvage IV (yeast) (4/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.49 or 2.7.4.7

Sequence Analysis Tools

See E0SN48 at UniProt or InterPro

Protein Sequence (267 amino acids)

>DDA3937_RS15335 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (Dickeya dadantii 3937)
MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGTSVITALVAQNTRGVQSVYRIEPAFV
AAQLDSVLSDVRIDSAKIGMLAQTDIVEAVADRLLRYPVPYVVLDTVMLAKSGDPLLAPE
AVEAVRQLLLPRVSLITPNLPEAAALLQCSPADDERGMRRQGDALLAMGCRAVLMKGGHL
SDSESPDWLFMPDAEPVRLSAPRVNTRHTHGTGCTLSAALAALRPRHDNWPDTLSAARAY
LQGALEQADSLEVGHGIGPVHHFHRWW