Protein Info for DDA3937_RS14995 in Dickeya dadantii 3937

Annotation: histidine ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 TIGR01225: histidine ammonia-lyase" amino acids 10 to 502 (493 residues), 690 bits, see alignment E=8.5e-212 PF00221: Lyase_aromatic" amino acids 15 to 473 (459 residues), 625.4 bits, see alignment E=3e-192

Best Hits

Swiss-Prot: 72% identical to HUTH_ACIAC: Histidine ammonia-lyase (hutH) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 100% identity to ddd:Dda3937_01661)

MetaCyc: 62% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SM85 at UniProt or InterPro

Protein Sequence (518 amino acids)

>DDA3937_RS14995 histidine ammonia-lyase (Dickeya dadantii 3937)
MSQTRSYCLTPGAVDLATLRAIYQGNVTLTLAEQARAAVTAAQETVQRIVRQDRVVYGIN
TGFGKLAQTRIPADRLAELQRNLVLSHSVGIGDLLPDDVLRLVMATKVVSLARGHSGVRL
QVIDTLLALFNAGVMPCIPEKGSVGASGDLAPLAHLSLLLLGEGPARVDGVLIPAAEGLA
RAGVEPLVLGPKEGLALLNGTQVSTALALRGLFEAEKVFAAGLVAGALSLEAIKGSVKPF
DARIHQARGQQGQMAVAAAVSTLLTDSEILQSHANCGRVQDPYSIRCVPQVMGACLDNLQ
HAARVLCIEANAASDNPLVFPETDEVISGGNFHAEPVAFAADIIALAVAEIGAISERRMA
LLLDSTLSGLPPFLVNDGGVNSGFMIAQVTAAALASENKSLAHPGSVDSLPTSANQEDHV
SMATYAARRLGSMCFNTATVVGIEAMAAAQGIDFHRPLKSSPLLERELAQVREQVAFLDH
DRLMATDIDLMRQWASRERWPSVITALLPSYAADPASV