Protein Info for DDA3937_RS14305 in Dickeya dadantii 3937

Annotation: NADH-quinone oxidoreductase subunit NuoH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00146: NADHdh
  • amino acids 18 to 317 (300 residues), 389.8 bits, E=4.4e-121

Best Hits

Swiss-Prot: 89% identical to NUOH_PECAS: NADH-quinone oxidoreductase subunit H (nuoH) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00337, NADH dehydrogenase I subunit H [EC: 1.6.5.3] (inferred from 100% identity to ddd:Dda3937_01802)

MetaCyc: 86% identical to NADH:quinone oxidoreductase subunit H (Escherichia coli K-12 substr. MG1655)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]; 7.1.1.- [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NADH to fumarate electron transfer (2/2 steps found)
• nitrate reduction VIII (dissimilatory) (2/2 steps found)
• NAD(P)/NADPH interconversion (4/6 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NADH to dimethyl sulfoxide electron transfer (1/2 steps found)
• NADH to hydrogen peroxide electron transfer (1/2 steps found)
• NADH to trimethylamine N-oxide electron transfer (1/2 steps found)
• aerobic respiration I (cytochrome c) (2/4 steps found)
• Fe(II) oxidation (1/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

See E0SKH8 at UniProt or InterPro

Protein Sequence (324 amino acids)

>DDA3937_RS14305 NADH-quinone oxidoreductase subunit NuoH (Dickeya dadantii 3937)
MNWLTPEVLDILATVGKAIVILLVVVSCGALMSMGERRLLALFQGRYGPNRVGWGGSLQL
VADMLKMFFKEDWTPPFADKVIFTLAPVIAFTSLLLSFAIVPVSPTWQVANLNIGVLFFL
MMAGLAVYAVLFAGWSSNNKYSLLGAVRASAQTLSYEVFLGLSLMGVVAQAGSFNMADIV
NAQSTVWNVVPQFFGFITFAIAGVAVCHRHPFDQPEAEQELADGYHIEYAGMKFGLFFVG
EYIGIVTVSALIVTLFFGGWHGPFLPPFVWFALKTAFFMMMFILIRASLPRPRYDQVMAF
GWRVCLPLTLLNLLATAAVILYNA