Protein Info for DDA3937_RS14230 in Dickeya dadantii 3937

Annotation: hydrogenase-2 assembly chaperone

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 169 transmembrane" amino acids 57 to 73 (17 residues), see Phobius details PF11939: NiFe-hyd_HybE" amino acids 18 to 161 (144 residues), 162.8 bits, see alignment E=3e-52 TIGR03993: [NiFe] hydrogenase assembly chaperone, HybE family" amino acids 22 to 160 (139 residues), 164.9 bits, see alignment E=5.3e-53

Best Hits

Swiss-Prot: 46% identical to HYBE_ECO57: Hydrogenase-2 operon protein HybE (hybE) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 99% identity to ddd:Dda3937_01818)

Predicted SEED Role

"Hydrogenase-2 operon protein hybE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SKG2 at UniProt or InterPro

Protein Sequence (169 amino acids)

>DDA3937_RS14230 hydrogenase-2 assembly chaperone (Dickeya dadantii 3937)
MPGERKESAWIEGHDQSPVAWLEAEFSRIAQERMRLLPFYRNGIPVRACGFTLFEQQWFG
CLLTPWMLSLLVLPGPGQQWPRRDLATRLALDLPCGSVKFVVSESDDGRQYLSCSLMSPL
DPALDAGQALQLAQQSARMALSLPVRDTDAPENLGRRALFSRYRSQRDA