Protein Info for DDA3937_RS14085 in Dickeya dadantii 3937

Annotation: nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 884 PF04879: Molybdop_Fe4S4" amino acids 5 to 52 (48 residues), 52.3 bits, see alignment 8.8e-18 PF00384: Molybdopterin" amino acids 67 to 475 (409 residues), 248.5 bits, see alignment E=2.1e-77 PF01568: Molydop_binding" amino acids 576 to 685 (110 residues), 93.8 bits, see alignment E=1.4e-30 PF04324: Fer2_BFD" amino acids 824 to 872 (49 residues), 49.6 bits, see alignment 8e-17

Best Hits

KEGG orthology group: K00372, nitrate reductase catalytic subunit [EC: 1.7.99.4] (inferred from 100% identity to ddd:Dda3937_01849)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SKC8 at UniProt or InterPro

Protein Sequence (884 amino acids)

>DDA3937_RS14085 nitrate reductase (Dickeya dadantii 3937)
MSGCRTTCPYCGVGCGVIATPQPDGRVTIEGDRQHPANLGRLCVKGSALGDTLDLQGRLL
WPLVDGHRVSWDQALDRVAQSLGDIIAQHGPQAVAFYGSGQLLTEDYYVANKLMKGFIGS
ANMDTNSRLCMASAVVGYKRALGADAVPCSYEDIEQADLVVLVGSNTAWAHPVVYQRLVQ
AKQQRPHMRVVVIDPRHTATCDAAELHLPLAPGSDAGLFNGLLSWLAQRNAPVPDGLSGV
EAALAAADDWTLERVAEFCQLDREDVACFYQWFADTDKVVTLYSQGINQSSSGSDKCNAI
INVHLFSGRIGRVGCGPFSITGQPNAMGGREVGGLANQLAAHMVFSPQEVDRVGRFWGSD
RVAPMPGLMAVDLFRAIEAGQVKAVWIMGTNPVVSMPEADRVRQALLRCPLVIVSDVMRH
TDTADCAHVLLPALAWGEKDGTVTNSERRISRQRAFLPAPGEAKADWWILSQVAQRMGFG
DAFDYRHPAQIFREHAALSGFENQEQRAFNISALATLSDEQWQQLTPVQWPVTANAGDGT
GRLYTDGRCWHLDGKARLLAITPQLPVNPPSSAYPLVLNTGRVRDQWHTMTRTGKAPRLM
RHLPEPYCDLHPQDALNAGVHDGELVRISAESGWMLARAQVQRGQQRGSIFVPMHWNRQF
SAQARADSLVAAVTDPYSGQPESKQARVRIQRWPAAWFGELFVRGDVTAPDCGYWSRIAA
DGVSHYTIAGEQTPDRWLDWLARQYGLGLDGVDYQQAQGDGGLFHAVGWLSGQVAVALYV
DVRRPALERETILAAFASPPQQTSDRLALLAGCAPQGEAPQGATICSCFAVGEQRIIDAI
RQGCHSVAQLGEQLQCGTNCGSCVPELKALLQQYATPDTLRRAG