Protein Info for DDA3937_RS13085 in Dickeya dadantii 3937

Annotation: metal-dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 264 TIGR00010: hydrolase, TatD family" amino acids 3 to 256 (254 residues), 304.9 bits, see alignment E=2.1e-95 PF01026: TatD_DNase" amino acids 4 to 255 (252 residues), 285.3 bits, see alignment E=2.2e-89

Best Hits

Swiss-Prot: 76% identical to YCFH_ECOLI: Uncharacterized metal-dependent hydrolase YcfH (ycfH) from Escherichia coli (strain K12)

KEGG orthology group: K03424, TatD DNase family protein [EC: 3.1.21.-] (inferred from 100% identity to ddd:Dda3937_02251)

Predicted SEED Role

"Putative deoxyribonuclease YcfH" in subsystem YcfH

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.-

Use Curated BLAST to search for 3.1.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SHP3 at UniProt or InterPro

Protein Sequence (264 amino acids)

>DDA3937_RS13085 metal-dependent hydrolase (Dickeya dadantii 3937)
MLLVDSHCHLDGLDYQSLHKDVSDVLNKAREREVGFFLAVATTLPGFRAMTELIGERDNV
AFSCGVHPLSQEEPYDFAELRTLAALPSVVALGETGLDYYYQQDTIPQQQASFREHIRVG
RALNKPVIVHTRSAREDTLAILREEKADECGGVLHCFTEDRETAEKLLDLGFYISFSGIV
TFRNADALREVARYVPLDRLLIETDSPWLAPVPYRGKENQPAYVRDVAEYLAVIKNVSLE
QLASTTTENFARLFHIDAARLTVA