Protein Info for DDA3937_RS12870 in Dickeya dadantii 3937

Annotation: FMNH2-dependent alkanesulfonate monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 TIGR03565: alkanesulfonate monooxygenase, FMNH(2)-dependent" amino acids 4 to 349 (346 residues), 630.4 bits, see alignment E=3.5e-194 PF00296: Bac_luciferase" amino acids 4 to 326 (323 residues), 290.6 bits, see alignment E=8.2e-91

Best Hits

Swiss-Prot: 80% identical to SSUD_ENT38: Alkanesulfonate monooxygenase (ssuD) from Enterobacter sp. (strain 638)

KEGG orthology group: K04091, alkanesulfonate monooxygenase [EC: 1.14.14.5] (inferred from 100% identity to ddd:Dda3937_03510)

MetaCyc: 78% identical to FMNH2-dependent alkanesulfonate monooxygenase (Escherichia coli K-12 substr. MG1655)
Alkanesulfonate monooxygenase. [EC: 1.14.14.5]; 1.14.14.5 [EC: 1.14.14.5]

Predicted SEED Role

"Alkanesulfonate monooxygenase (EC 1.14.14.5)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.14.14.5)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.14.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SGW1 at UniProt or InterPro

Protein Sequence (382 amino acids)

>DDA3937_RS12870 FMNH2-dependent alkanesulfonate monooxygenase (Dickeya dadantii 3937)
MSLNIFWFLPTHGDGHYFGKGENARPVDYGYLQQIAQAADRLGFGGVLIPTGRSCEDSWL
VASSLIPVTQRLRFLVALRPGIISPTLAARQAATLDRLSNGRALFNLVTGGDPDELAAEG
LFLDHAERYEASAEFTRIWRRVLEGETVDYDGKHIQVKGAKLHYPPVQQPRPPLYFGGSS
DAALDLAAEQVELYLTWGEPPAQVKEKLDRVRAKAAEQGRSVRFGIRLHVIVRETNEEAW
QAANRLISRLDDDTIAQAQASLKRFDSVGQQRMVALHGGRRDKLEISPNLWAGIGLVRGG
AGTALVGDPQTVAQRIQEYADLGIDTFVLSGYPHLEEAYKVGELLFPHLDVAVPEVPQPQ
PLRSQGEIVGNEFVPLLKAAQS