Protein Info for DDA3937_RS12855 in Dickeya dadantii 3937

Annotation: quinone-dependent dihydroorotate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 2 to 335 (334 residues), 523.9 bits, E=7.8e-162
• PF01180: DHO_dh
  • amino acids 46 to 334 (289 residues), 289.6 bits, E=1.3e-90

Best Hits

Swiss-Prot: 90% identical to PYRD_PECCP: Dihydroorotate dehydrogenase (quinone) (pyrD) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 100% identity to ddd:Dda3937_03514)

MetaCyc: 83% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Sequence Analysis Tools

See E0SGV7 at UniProt or InterPro

Protein Sequence (335 amino acids)

>DDA3937_RS12855 quinone-dependent dihydroorotate dehydrogenase (Dickeya dadantii 3937)
MYPLIRKALFQLDPEQAHEFTLHQLRRITHTPFEWLVRQSVPTKPVTCMGLSFRNPLGLA
AGLDKDGECIDALGAMGFGFIEVGTVTPRPQPGNDKPRLFRVVEAEGLINRMGFNNRGVD
NLVENVKQSRFGGVLGINIGKNKDTPVEQGKDDYLICMDKVYSHAGYIAINISSPNTPGL
RTLQYGDALDDLLQAVKNKQTELHTRYQKYVPVAVKIAPDLSEEELIQIADSLVRHNIDG
VIATNTTLDRQLIQGLNHCGQTGGLSGRPLQSRSTEVIRRLSQELQGRLPIIGVGGIDSV
VAAREKLAAGATLVQIYSGFIYRGPRLIKDIVTHI