Protein Info for DDA3937_RS12570 in Dickeya dadantii 3937

Annotation: tyrosine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 TIGR00234: tyrosine--tRNA ligase" amino acids 1 to 424 (424 residues), 476 bits, see alignment E=5.3e-147 PF00579: tRNA-synt_1b" amino acids 31 to 328 (298 residues), 305.6 bits, see alignment E=3.6e-95 PF01479: S4" amino acids 359 to 403 (45 residues), 27.2 bits, see alignment 2.6e-10

Best Hits

Swiss-Prot: 91% identical to SYY_KLEP7: Tyrosine--tRNA ligase (tyrS) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 100% identity to ddd:Dda3937_00065)

MetaCyc: 89% identical to tyrosine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Tyrosine--tRNA ligase. [EC: 6.1.1.1]; 6.1.1.1 [EC: 6.1.1.1]

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SG20 at UniProt or InterPro

Protein Sequence (425 amino acids)

>DDA3937_RS12570 tyrosine--tRNA ligase (Dickeya dadantii 3937)
MMASNLIQQLQERGLVAQVTDEGALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
FQMAGHKPVALVGGATGLIGDPSFKAAERKLNTEDTVQEWVEKIRRQAAPFLDFDCGDNS
AVAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDVGISFTEFSYNLLQG
YDFASLNALHGVELQIGGSDQWGNITSGIDLTRRLHQKQVFGLTVPLITKSDGTKFGKTE
SGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFLDIETINALEEEDKNSGAAPRAQ
YVLAEEVTRLVHGEEGLTAAKRITQSLFNGNLSDLTEADFAQLAQDGVPMVELATGADLQ
QALVDSELQPSRGQARKTIASNAITINGEKQSDPEYTFTGADRLFGRYTLLRRGKKNYCL
VCWKD