Protein Info for DDA3937_RS10695 in Dickeya dadantii 3937

Annotation: bifunctional NADP-dependent 3-hydroxy acid dehydrogenase/3-hydroxypropionate dehydrogenase YdfG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 2 to 187 (186 residues), 181.3 bits, E=3.9e-57
• PF01370: Epimerase
  • amino acids 3 to 135 (133 residues), 26.1 bits, E=1.4e-09
• PF08659: KR
  • amino acids 4 to 158 (155 residues), 45.1 bits, E=2.7e-15
• PF13561: adh_short_C2
  • amino acids 7 to 220 (214 residues), 122.4 bits, E=5.9e-39
• PF03807: F420_oxidored
  • amino acids 12 to 48 (37 residues), 22.1 bits, 4.7e-08

Best Hits

Swiss-Prot: 75% identical to YDFG_SALTI: NADP-dependent 3-hydroxy acid dehydrogenase YdfG (ydfG) from Salmonella typhi

KEGG orthology group: None (inferred from 100% identity to ddd:Dda3937_04134)

MetaCyc: 76% identical to 3-hydroxy acid dehydrogenase YdfG (Escherichia coli K-12 substr. MG1655)
RXN-16000 [EC: 1.1.1.381]; RXN-8974 [EC: 1.1.1.381, 1.1.1.298]; Serine 3-dehydrogenase. [EC: 1.1.1.381, 1.1.1.298, 1.1.1.276]

Predicted SEED Role

"3-hydroxypropionate dehydrogenase (EC 1.1.1.298)" (EC 1.1.1.298)

MetaCyc Pathways

• superpathway of L-threonine metabolism (14/18 steps found)
• L-threonine degradation III (to methylglyoxal) (2/3 steps found)
• β-alanine degradation III (1/2 steps found)
• uracil degradation III (3/5 steps found)
• aminopropanol phosphate biosynthesis II (2/4 steps found)
• 3-hydroxypropanoate cycle (6/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (6/18 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.276 or 1.1.1.298 or 1.1.1.381

Sequence Analysis Tools

See E0SC08 at UniProt or InterPro

Protein Sequence (249 amino acids)

>DDA3937_RS10695 bifunctional NADP-dependent 3-hydroxy acid dehydrogenase/3-hydroxypropionate dehydrogenase YdfG (Dickeya dadantii 3937)
MIIFVTGATAGFGEAITRRFIQEGHQVIATGRRQERLDALKAEFGAALLTLRLDVRDRQA
IEQAIGSLSAEWRNIDVLVNNAGLALGLEPAHKASVSDWETMIDTNNKGLVFMTHALLPE
MVKRNVGHIINIGSTAGNWPYLGGNVYGASKAFVRQFSLGLRADLSGTRVRVTNIEPGLV
GGTEFSAVRFKGDDAKVSQTYDKSNPLTPEDVTEAVFWVATLPAHVNINTLEMMPVSQTY
GGLKVNRDE