Protein Info for DDA3937_RS10080 in Dickeya dadantii 3937

Annotation: Holliday junction branch migration protein RuvA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 205 PF01330: RuvA_N" amino acids 1 to 62 (62 residues), 77.9 bits, see alignment E=1.3e-25 TIGR00084: Holliday junction DNA helicase RuvA" amino acids 1 to 205 (205 residues), 227.2 bits, see alignment E=6.2e-72 PF14520: HHH_5" amino acids 72 to 130 (59 residues), 58.7 bits, see alignment E=1.6e-19 PF07499: RuvA_C" amino acids 160 to 203 (44 residues), 60.4 bits, see alignment 4.8e-20

Best Hits

Swiss-Prot: 84% identical to RUVA_PHOLL: Holliday junction ATP-dependent DNA helicase RuvA (ruvA) from Photorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)

KEGG orthology group: K03550, holliday junction DNA helicase RuvA (inferred from 100% identity to ddd:Dda3937_01621)

MetaCyc: 79% identical to Holliday junction branch migration complex subunit RuvA (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvA" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SAW6 at UniProt or InterPro

Protein Sequence (205 amino acids)

>DDA3937_RS10080 Holliday junction branch migration protein RuvA (Dickeya dadantii 3937)
MIGRLRGIVLEKQPPLVLIEASGVGYEVHMPMTCFYELPDLGQEAVIFTHFVVREDAQLL
FGFNNKQERSLFRELIKVNGVGPKLALAILSGMSAQQFVSAVERQEINALVKLPGVGKKT
AERLVVEMKDRFKGLNGDLFNAASDIALPEAAGKAEPEADPQAEAEAALVALGYKPQEAG
RMVSKVARPGADCETLIREALRAAL