Protein Info for DDA3937_RS10055 in Dickeya dadantii 3937

Annotation: aspartate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 596 TIGR00459: aspartate--tRNA ligase" amino acids 1 to 584 (584 residues), 898.5 bits, see alignment E=8.8e-275 PF01336: tRNA_anti-codon" amino acids 18 to 101 (84 residues), 57.1 bits, see alignment E=2.1e-19 PF00152: tRNA-synt_2" amino acids 117 to 557 (441 residues), 412.4 bits, see alignment E=1.6e-127 PF02938: GAD" amino acids 307 to 406 (100 residues), 99 bits, see alignment E=2.2e-32

Best Hits

Swiss-Prot: 89% identical to SYD_PECAS: Aspartate--tRNA ligase (aspS) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 100% identity to ddd:Dda3937_01616)

MetaCyc: 82% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SAW1 at UniProt or InterPro

Protein Sequence (596 amino acids)

>DDA3937_RS10055 aspartate--tRNA ligase (Dickeya dadantii 3937)
MRTEYCGLLNSSHVGQEVTLCGWVNRRRDLGGLIFIDMRDREGLVQVFFDPDRQDAFTLA
SELRNEFCIQLTGVVRARPDNQVNKEMATGAVEVLATALTIINRAEPLPLDANQTNSEEA
RLKYRYLDLRRPEMAQRLKTRARITSFVRRFMDSHGFLDIETPMLTKATPEGARDYLVPS
RVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMT
APQVREVMEKLVRELWLEIHGVDLGDFPIMTFAEAMRRFGSDKPDLRNPLELVDVADLLK
DVDFKVFSGPANDPKGRVAAIRVPGGAQLSRKQIDEYAKFIEIYGAKGLAYIKVNEKDNG
LEGIQSPVAKFLNDGILSALLARTAAEDGDILFFGADSAKVVTDALGALRLKLGRDLKIT
NENSWQPLWVIDFPMFEEDGEGGLAAMHHPFTAPRDMSPTELEANPTAAIANAYDMVLNG
YEVGGGSVRIHRGEMQQTVFRILGIDERQQREKFGFLLDALKFGTPPHAGLAFGLDRLVM
LLTGTDNIRDVIAFPKTTAAACLMTEAPSFANPSALEELSIAVVGKGKAAQDAENQ