Protein Info for DDA3937_RS09220 in Dickeya dadantii 3937

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01891: amidohydrolase" amino acids 47 to 395 (349 residues), 264.5 bits, see alignment E=7.8e-83 PF01546: Peptidase_M20" amino acids 101 to 405 (305 residues), 86 bits, see alignment E=3.6e-28 PF07687: M20_dimer" amino acids 222 to 316 (95 residues), 44.7 bits, see alignment E=1.1e-15

Best Hits

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 100% identity to ddd:Dda3937_03627)

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32, 3.5.1.47

Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SM03 at UniProt or InterPro

Protein Sequence (445 amino acids)

>DDA3937_RS09220 amidohydrolase (Dickeya dadantii 3937)
MKLNSALCLIMSMNYLSNVAYAFDIQKASKATDSYLDINYSQLDNLYKDIHQNPELGYQE
VRTTKILANNMRKIGFQVMDNLGGTGFVAIYKNGDGPTVMVRTELDALPMQEKTGLPYAS
TVKQKDQDGKETYVAHSCGHDIHMASWFGSASALINMKEQWQGTLMFVAQPAEEKITGAS
AMVNDGIFKKFGKPDYAFALHTSPMEYGFVSFKPGVQTSNGDSFSVTFKGKGGHGSMPEK
TIDPIVIAARFVTDVQTLISRETSPNKFGVVTIGAFNSGTSGNIIPDMAKIQGTLRTYDD
NVRKNLIDGIARFAKASADMSKAPLPEIIIGENKVDSIVNDSSLSESTAKVFKERFGNHF
TEAKEPSSASEDFSVFVNAGIPAVYFNIGIYSPEQIKQWEREGVEIPSNHSPLFAPVPEP
TIRTGVEAMTLAVMNALDNNVGKKL