Protein Info for DDA3937_RS08920 in Dickeya dadantii 3937

Annotation: putative acyl-CoA thioester hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01095: Pectinesterase
  • amino acids 198 to 360 (163 residues), 58.2 bits, E=2.9e-20

Best Hits

Swiss-Prot: 100% identical to PMEB_DICD3: Pectinesterase B (pemB) from Dickeya dadantii (strain 3937)

KEGG orthology group: K01051, pectinesterase [EC: 3.1.1.11] (inferred from 100% identity to ddd:Dda3937_03435)

Predicted SEED Role

"Pectinesterase precursor (EC 3.1.1.11)" (EC 3.1.1.11)

MetaCyc Pathways

• pectin degradation I (3/3 steps found)
• pectin degradation II (3/5 steps found)

KEGG Metabolic Maps

• Pentose and glucuronate interconversions
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.11

Use Curated BLAST to search for 3.1.1.11

Sequence Analysis Tools

See Q47474 at UniProt or InterPro

Protein Sequence (433 amino acids)

>DDA3937_RS08920 putative acyl-CoA thioester hydrolase (Dickeya dadantii 3937)
MSLTHYSGLAAAVSMSLILTACGGQTPNSARFQPVFPGTVSRPVLSAQEAGRFTPQHYFA
HGGEYAKPVADGWTPTPIDTSRVTAAYVVGPRAGVAGATHTSIQQAVNAALRQHPGQTRV
YIKLLPGTYTGTVYVPEGAPPLTLFGAGDRPEQVVVSLALDSMMSPADYRARVNPHGQYQ
PADPAWYMYNACATKAGATINTTCSAVMWSQSNDFQLKNLTVVNALLDTVDSGTHQAVAL
RTDGDRVQLENVRLLSRQDTFFVNTSDRQNSYVTDHYSRAYIKDSYIEGDVDYVFGRATA
VFDRVRFHTVSSRGSKEAYVFAPDSIPSVKYGFLVINSQLTGDNGYRGAQKAKLGRAWDQ
GAKQTGYLPGKTANGQLVIRDSTIDSSYDLANPWGAAATTDRPFKGNISPQRDLDDIHFN
RLWEYNTQVLLHE