Protein Info for DDA3937_RS08510 in Dickeya dadantii 3937

Annotation: biotin synthase BioB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 345 TIGR00433: biotin synthase" amino acids 14 to 310 (297 residues), 410.8 bits, see alignment E=1.6e-127 PF04055: Radical_SAM" amino acids 48 to 205 (158 residues), 82.9 bits, see alignment E=3.1e-27 PF06968: BATS" amino acids 220 to 311 (92 residues), 106.5 bits, see alignment E=6.7e-35

Best Hits

Swiss-Prot: 91% identical to BIOB_PECAS: Biotin synthase (bioB) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K01012, biotin synthetase [EC: 2.8.1.6] (inferred from 100% identity to ddd:Dda3937_03748)

MetaCyc: 84% identical to biotin synthase (Escherichia coli K-12 substr. MG1655)
Biotin synthase. [EC: 2.8.1.6]

Predicted SEED Role

"Biotin synthase (EC 2.8.1.6)" in subsystem Biotin biosynthesis (EC 2.8.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SK91 at UniProt or InterPro

Protein Sequence (345 amino acids)

>DDA3937_RS08510 biotin synthase BioB (Dickeya dadantii 3937)
MTERVNWTLEQAQALFDKPFLELMFEAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYC
PQSARYRTGIETERLMQVEQVLDSARQAKAAGSTRFCMGAAWKNPHERDMPYLESMVRGV
KEMGMETCMTLGTLHGDQAERLAAAGLDFYNHNLDTSPEFYGNIITTRTYQERLDTLGKV
RGAGIKVCSGGIVGLGETVRDRAGLLVQLANLPTPPESVPINMLVKVKGTPLAETEDVDP
FDFIRTIAVARIMMPASYVRLSAGREQMSEQTQAMCFMAGANSIFYGCKLLTTPNPKEDK
DLQLFRKLGLNPQQTQTEYGDNQQQQHLAERLLHADTEHYYNAAV