Protein Info for DDA3937_RS07470 in Dickeya dadantii 3937

Annotation: hydrogenase 4 subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 679 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 44 to 68 (25 residues), see Phobius details amino acids 88 to 111 (24 residues), see Phobius details amino acids 118 to 137 (20 residues), see Phobius details amino acids 143 to 161 (19 residues), see Phobius details amino acids 173 to 194 (22 residues), see Phobius details amino acids 214 to 236 (23 residues), see Phobius details amino acids 250 to 268 (19 residues), see Phobius details amino acids 279 to 302 (24 residues), see Phobius details amino acids 314 to 335 (22 residues), see Phobius details amino acids 351 to 370 (20 residues), see Phobius details amino acids 391 to 411 (21 residues), see Phobius details amino acids 433 to 458 (26 residues), see Phobius details amino acids 480 to 504 (25 residues), see Phobius details amino acids 540 to 559 (20 residues), see Phobius details amino acids 659 to 678 (20 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 144 to 427 (284 residues), 187.7 bits, see alignment E=1.4e-59

Best Hits

Swiss-Prot: 61% identical to HYFB_ECOLI: Hydrogenase-4 component B (hyfB) from Escherichia coli (strain K12)

KEGG orthology group: K12137, hydrogenase-4 component B [EC: 1.-.-.-] (inferred from 100% identity to ddd:Dda3937_00728)

Predicted SEED Role

"Hydrogenase-4 component B"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SHL2 at UniProt or InterPro

Protein Sequence (679 amino acids)

>DDA3937_RS07470 hydrogenase 4 subunit B (Dickeya dadantii 3937)
MMTPLQLFTPLQWLGLSLALYLAGALLSLCLCRQESLAIRLSGLCALAGGCAGALAAAPV
LLSGGVLTAVFDGPFDAFAHLTLRFDTLAAFMTLVISLLVAVSALYSLAYLEEYRGRGAW
AMGFFMNLFVASMVALVVVDNAFYFIVLFEVMSLSSYFLVIADQDEEAVGAGLLYFLIAH
AGSVLIMIAFFLLYRHSSSLDFADFRHAGLSAPQASVVFLLAFFGFGAKAGMLPLHGWLP
RAHPAAPSHASALMSGVMVKIGVFGIIKVGVDLLGATETWWGVVVLAFGAVSSVLGVLYA
LAEHDIKRLLAYHTVENIGIILMGVGVGMIGIATHHPLLALLGLLGGLYHLLNHAVFKGL
LFLGAGAVIYRVHTKDMEKMGGLSRLMPRTALAFLVGCMAISALPPLNGFVSEWFTYQSL
FTLSHDGGIGLRLAAPIAIVMLAITGALAAMCFVKVYGISFCGAPRSEKAAHAREVPWPM
TLAMLLLALLCVLLGVGASVVVPVMTRIAADLAHTPEVMMAAGLGVFPGDHLQTRLNTPL
IFILLLALPLLPLMLYSLLRGPRLGFRHKGTPWACGYGYESAMSASAGSFTQPLRVMFAP
LYRVRKTLDPAPTMQQALEKTTQAAARVEPVWDDHVVMPVVSGVQRISRAVQWIQHGDFR
VYCLYVVAALVALLLATLA